Journal of Ecophysiology and Occupational Health

N. Shahi ¹, S. K. Mallik ¹, D. Sarma ¹

Affiliations
1 Directorate of Coldwater Fisheries Research (ICAR), Bhimtal 263 136, Nainital, (UK), IN

Abstract

Total and differential leukocyte count, and the phagocytic activity in blood of common carp, Cyprinus carpio (Linnaeus, 1758), experimentally infected with virulent Aeromonas allosaccharophila was evaluated in this study. For experimental study, common carp fingerlings (10.0 ± 1.5 g Weight, 8.5 ± 2.3 cm Length) were divided into five groups in duplicates of un-injected fish, fish injected with sterile phosphate buffered saline (PBS), and fish injected with 4.2 x 104, 1.9 x 106 and 7.3 x 108 colony forming units per milliliter (CFU ml-1) of suspension of A. allosaccharophila. Forty eight hours after infection, the total and differential leukocyte count and phagocytic activity of blood leukocytes of common carp was determined. The leukocyte, lymphocyte and monocyte count were significantly (P< 0.05) high in experimentally infected fishes, while no significant change in neutrophils number was observed. In fishes injected with 4.2 x 104, 1.9 x 106 and 7.3 x 108 CFU ml-1 of bacterial suspension, the percentage of phagocytic activity in the blood were 55.3, 54.7 and 58.2, respectively. The result suggests that, A. allosaccharophila could influence the leukocyte number and phagocytic activity of blood of common carp, which is an integral component of innate immune system of fish.

Keywords

Bacteria, Blood, Fish, Innate Immunity, Phagocytosis.

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N. Shahi ¹, S. K. Mallik ¹, D. Sarma ¹, M. Sahoo ², P. Das ¹

Affiliations
1 Directorate of Coldwater Fisheries Research, (ICAR) Industrial Area, Bhimtal, 263 136, Nainital, (Uttarakhand), IN
2 Indian Veterinary Research Institute, Izatnagar, Bareilly, (UP), IN

Abstract

Corneal hypertrophy was observed in fingerlings of chocolate mahseer, Neolissochilus hexagonolepis (McClelland, 1839) from Uttarakhand, India. The advanced stage of infection was marked with damage of cornea and shrunken eye. Skeletal deformity was also recorded in some of the infected fishes. Samples of eye and other organ were collected for histopathological, parasite and microbial investigation. Histological examination of the eye indicated ocular collapse, entirely damaged cornea, disruption of vitreous, aphakic globe, oedema of choroid and optic nerve and, deformed retina. Microscopic observations of wet mounts and histopathology ruled out infectious agents like microsporidia, myxosporidea, cystercoidiasis, atypical mycobacteria, digeneans and fungi. PCR for viral aetiology did not yield any significant amplicons. Further detail study related to physic-chemical parameter need to be carried out to ascertain the exact cause of this disease.

Keywords

neolissochilus Hexagonolepis, Pathology, Eye, Corneal Hypertrophy, Chocolate Mahseer.

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N. Shahi ¹, J. Pandey ¹, S. K. Mallik ¹, D. Sarma ¹, P. Das ¹

Affiliations
1 Industrial Area, Bhimtal, Uttarakhand-263 136, IN

Abstract

Golden mahseer, Tor putitora is an endangered fish of mid-Himalayan regions of India. It has good potential as aquaculture species for hill aquaculture, due to its high market demand. But the major constrains on introduction of this fish for aquaculture is its inability to breed in captivity and slow growth rate. Therefore, studies were carried out on its breeding biology to captive breed this fish for sustainable seed production. However, detailed study on reproductive pattern of adult golden mahseer is very limited. Therefore, in the present study, we have developed a macroscopic grading system for golden mahseer testes, which shows the testes development stages during the breeding season, which lasts from May-September in Bhimtal and Sattal lakes. The golden mahseer were sampled from February - September 2014, from Bhimtal and Sattal lakes. Altogether, 48 samples were collected during the sampling period. Based on macroscopic observation, the maturation stage of the testes was divided into five developmental stages: Immature (I), early spermatogenic (II), late spermatogenic (III), matured (IV) and spent (V).

Keywords

Tor Putitora, Reproduction, Maturity, Testes.

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