Whey Protein and Nigella sativa Oil Mitigate Potassium Dichromate Induced Hepatic Injury, Oxidative Stress and Hematotoxicity in Rats
The present study was carried out to evaluate the antioxidant property of whey protein and/or Nigella sativa Oil (NSO) against hepatotoxicity evoked by potassium dichromate(K2Cr2O7). Designed for this purpose, we detected the 8 weeks challenge result of whey protein (100 and 200 mg/kg, p.o) with/or without Nigella sativa oil (5ml/kg, p.o) in contradiction of poisoned albino rats with one dose of potassium dichromate (30mg/Kg, I.P) at the end of challenge period. Concerning plasma level, whey protein with/or without Nigella sativa oil were ameliorated the potassium dichromate liver damage concerns, so it exhibited a major progress in Aspartate Aminotransferase (AST), Alanine Amino Transferase (ALT), Alkaline Phosphatase (ALP), and Gamma-Glutamyl Transferase (GGT). Moreover, whey protein or Nigella sativa oil reduce the deleterious effects of potassium dichromate on Triiodothyronine (T3), Thyroxine (T4), Thyroidstimulating Hormone (TSH), glucose and Complete Blood Count (CBC). In addition, they displayed an important improvement in hepatic antioxidant enzymes, Catalase (CAT) and Superoxide Dismutase (SOD) beside reduced Glutathione (GSH), and with a subsequent decrease in Malondialdehyde (MDA) or Nitric Oxide (NO) levels in comparison with the untreated K2Cr2O7 group. Also, whey protein with/or without Nigella sativa oil improve the histopathological alterations produced by the potassium dichromate. These outcomes suggest that whey protein or Nigella sativa oil can be used as effective antioxidant aginst potassium dichromate intoxication as they modulate liver function and decrease oxidative stress.
- Gu X, Manautou JE. Molecular mechanisms underlying chemical liver injury. Expert Rev Mol Med. 2012; 14:1-22. https://doi.org/10.1017/S1462399411002110 PMid:22306029 PMCid:PMC3704158
- Levis AG, Buttignol M, Vettorato L. Inhibition of DNA synthesis in BHK fibroblasts treated in vitro with potassium dichromate. Experientia. 1977; 33(1):82–4. https://doi.org/10.1007/BF01936767 PMid:836428
- Niki E, Noguchi N. Dynamics of antioxidant action of vitamin E. Acc Chem Res. 2004; 37(1):45–51. https://doi.org/10.1021/ar030069m PMid:14730993
- Safaeian L, Zabolian H. Antioxidant effects of bovine lactoferrin on Dexamethasone-Induced Hypertension in Rat. ISRN Pharmacology. 2014.p. 1-6.
- Begum NA, Dewan ZF, Nahar N, Rouf Memun MI. Effect of n-Hexane extract of Nigella sativa on gentamicin induced nephrotoxicity in rats. Bangladesh J Pharmacol. 2006; 1(1):16–20.
- Mahmood T, Qureshi IZ, Iqbal MJ. Histopathological and biochemical changes in rat thyroid following acute exposure to hexavalent chromium. Histol Histopathol. 2010; 25(11):1355–70. PMid:20865659
- Eliwa HA, El-Denshary ES, Nada SA, Elyamany MF, Omara EA, Asaaf N. Evaluation of the therapeutic effect of whey proteins on the hepatotoxicity induced by paracetamol and alcohol coadministration in rats. Int J Pharm Res Bio-Sci. 2014; 3(2):295–314.
- Develi S, Evran B, Betul Kalaz E, Kocak-Toker N, Erata GO. Protective effect of Nigella sativa oil against binge ethanolinduced oxidative stress and liver injury in rats. Chin J Nat Med. 2014; 12(7):495–9. https://doi.org/10.1016/S18755364(14)60077-7
- Attia, AM, El-Hefnawy AM, Conformational stability against auto-oxidation for mice and human oxyhemoglobins. Romanian J Biophys., 2009; 19(3):187–98.
- Stohs SJ, Bagchi D, Hassoun E, Bagchi M. Oxidative mechanisms in the toxicity of chromium and cadmium ions. J Environ Pathol Toxicol Oncol. 2000; 19(3):201–13. PMid:10983887
- Franchini I, Cavatorta A, D’Errico M, De Santis M, Romita G, Gatti R, et al. Studies on the etiology of the experimental neuropathy from industrial adhesive (glues). Experientia. 1978; 34(2):250-2. https://doi.org/10.1007/BF01944708 PMid:624366
- Norseth T. The carcinogenicity of chromium. Environ Health Perspect. 1981; 40:121–30. https://doi.org/10.1289/ ehp.8140121 PMid:7023928 PMCid:PMC1568823
- Patlolla AK, Barnes C, Hackett D, Tchounwou PB. Potassium dichromate induced cytotoxicity, genotoxicity and oxidative stress in human liver carcinoma (HepG2) cells. Int J Environ Res Public Health. 2009; 6(2):643–53. https://doi.org/10.3390/ijerph6020643 PMid:19440407 PMCid:PMC2672371
- Bagchi D, Vuchetich PJ, Bagchi M, Hassoun EA, Tran MX, Tang L, et al. Induction of oxidative stress by chronic administration of sodium dichromate [chromium VI] and cadmium chloride [cadmium II] to rats. Free Radic Biol Med. 1997; 22(3):471–8. https://doi.org/10.1016/S08915849(96)00352-8
- Nordberg J, Arner ES. Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radic Biol Med. 2001; 31(11):1287–312. https://doi.org/10.1016/ S0891-5849(01)00724-9
- Stift A, Friedl J, Langle F, Berlakovich G, Steininger R, Muhlbacher F. Successful treatment of a patient suffering from severe acute potassium dichromate poisoning with liver transplantation. Transplantation. 2000; 69(11):2454–5. https://doi.org/10.1097/00007890-200006150-00044 PMid:10868660
- Wang BJ, Sheu HM, Guo YL, Lee YH, Lai CS, Pan MH, et al. Hexavalent chromium induced ROS formation, Akt, NF-kappaB, and MAPK activation, and TNF-alpha and IL-1alpha production in keratinocytes. Toxicol Lett. 2010; 198(2):216–24. https://doi.org/10.1016/j.toxlet.2010.06.024 PMid:20619327
- Amacher DE. Serum transaminase elevations as indicators of hepatic injury following the administration of drugs. Regul Toxicol Pharmacol. 1998; 27(2):119–30. https://doi.org/10.1006/rtph.1998.1201 PMid:9671567
- Gueniche A, Viac J, Lizard G, Charveron M, Schmitt D. Effect of various metals on intercellular adhesion molecule-1 expression and tumour necrosis factor alpha production by normal human keratinocytes. Arch Dermatol Res. 1994; 286(8):466–70. https://doi.org/10.1007/BF00371573 PMid:7864660
- Lu SC, Bao Y, Huang ZZ, Sarthy VP, Kannan R. Regulation of gamma-glutamylcysteine synthetase subunit gene expression in retinal Muller cells by oxidative stress. Invest Ophthalmol Vis Sci. 1999; 40(8):1776–82. PMid:10393048
- Gurer H, Ozgunes H, Neal R, Spitz DR, Ercal N. Antioxidant effects of N-acetylcysteine and succimer in red blood cells from lead-exposed rats. Toxicology. 1998 Jul 17; 128(3):181– 9. https://doi.org/10.1016/S0300-483X(98)00074-2
- Hesham A Eliwa, Ezzedin S El-Denshary, Somaia A Nada, Gamal Elsherbini and Naglaa Asaaf. Antinociceptive effect of whey protein and its fractions in Swiss Albino mice. IJPRBS. 2012; 1(6):355–81.
- Dickinson DA, Forman HJ. Cellular glutathione and thiols metabolism. Biochem Pharmacol. 2002; 64(5-6):1019–26. https://doi.org/10.1016/S0006-2952(02)01172-3
- Griffith OW. Biologic and pharmacologic regulation of mammalian glutathione synthesis. Free Radic Biol Med. 1999; 27(9-10):922–35. https://doi.org/10.1016/S08915849(99)00176-8
- Boskabadi MH, Kiani S, Jandaghi P, Ziaei T, Zarei A. Comparison of antitussive effect of Nigella sativa with codeine in Guinea Pig. Iran J Med Sci. 2003; 28(3):111–5.
- Dinagaran S, Sridhar S, Eganathan P. Chemical composition and antioxidant activities of black seed oil (Nigella sativa L). Inter J Pharma Sci Res. 2016; 7(11):4473–9.
- Al-Seeni MN, El Rabey HA, Zamzami MA, Alnefayee AM. The hepatoprotective activity of olive oil and Nigella sativa oil against CCl4 induced hepatotoxicity in male rats. BMC Complement Altern Med. 2016; 16:1–14. https://doi.org/10.1186/s12906-016-1422-4 PMid:27814700 PMCid:PMC5097446
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