Estimation of Gatifloxacin in Pharmaceutical Dosage Forms by High Performance Liquid Chromatography

A simple, rapid, sensitive and precise High Performance Liquid Chromatographic (HPLC) method has been developed for the estimation of gatifloxacin in its pharmaceutical dosage forms. In this method RP - C ₁₈ column (150mmx4.6mm I.D., 5 μm particle size) with mobile phase consisting of acetonitrile and 0.05M phosphate buffer in the ratio of 25:75 v/v in isocratic mode was used. The detection wavelength is 293nm and the flow rate is 0.8ml/min. Ciprofloxacin is used as internal standard. The linearity was fo und to be in the range of 0.1 to 10 μg/ml and shows a correlation coefficient of 0.9999. The intra - and inter - day variation was found to be less than 1.2% showing high precision of the assay method. The mean percent recovery of the drug from the solution c ontaining 4 μg/ml was 100.5 ± 2.06 indicating high accuracy of the proposed method. Hence, this method is simple, fast, specific, accurate, precise and less time consuming for the estimation of gatifloxacin in pharmaceutical dosage forms.