Antibacterial and Antifungal Activity of Streptomyces Sp. VITTDK3 Isolated from Ennore Coast, Tamil Nadu, India

Actinobacteria isolated from soil samples collected at the Ennore saltern, Tamil Nadu was screened for antibacterial and antifungal activity against common clinical pathogens. The isolate VITDDK3 exhibited more promising antagonistic activity against Escherichia coli, Pseudomonas aeruginosa, Aspergillus flavus and Aspergillus fumigatus. Presence of LL-Diaminopimelic acid and glycine assigned the isolate VITDDK3 to the genus Streptomyces. Further 16S rRNA partial gene sequence was carried out to confirm the chemotaxonomic results. BLAST analysis showed that the strain VITDDK3 possessed 98% similarity with Streptomyces sp. A254Ydz-ZZ but phylogenetic analysis revealed that its closest neighbour was Streptomyces tricolor HBUM175176. The rRNA secondary structure and the restriction sites of VITDDK3 were predicted using Genebee and NEBCutter online softwares respectively.