Analytical Method Development and Validation for Dipyridamole

A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for dipyridamole in presence of its degradation products is developed and validated. The method employed Targa C₈ column i.e., (250 X 4.6 mm 5 μm particle size) column as stationary phase. The mobile phase consists of acetonitrile and pH3.0 buffer in the ratio 35:65 %. It is pumped through the chromatographic system at a flow rate of 1.2 ml /min. The UV detector is operated at 282 nm. This system was found to give good resolution between dipyridamole and its degradation products. Method was validated as per ICH guidelines.