In vitro  Shoot Regeneration Studies in  Java citronella

Bhawana R. Bobade; S. R. Kamdi; V. S. Wadhai

In vitro Shoot Regeneration Studies in Java citronella

Bhawana R. Bobade ¹, S. R. Kamdi ², V. S. Wadhai ³

Affiliations
1 Department of Biotechnology, Sardar Patel Mahavidyalaya, Chandrapur (M.S.), India
2 Anand Niketan College of Agriculture, Warora (M.S.), India
3 Department of Microbiology Sardar Patel College, Chandrapur (M.S.), India

Java citronella (Cymbopogon winterianus Jowitt.) is a aromatic plant of great economic value and also well known throughout the world due to its repellent properties attributed to its essential oil, rich in citronellal. An efficient protocol for rapid regeneration of Java citronella through callus was developed by manipulating growth regulators in the culture medium. Shoot tips and leaves were used as explants source for regeneration and callus induction. The best callus induction was achieved on MS medium supplemented with 2, 4-D 2.5 mg/L. The best rate of shoot regeneration was obtained on MS medium supplemented with BAP 2 mg/L. The multiplication of shoots was observed on MS medium supplemented with BAP 4 mg/L.