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Identification of Candidate Genes for Bacterial Leaf Blight Resistance in Rice by Integration of Genetic QTL Map with the Physical Map


Affiliations
1 National Research Center on Plant Biotechnology, IARI, New Delhi, India
2 Dept. of Boitechnology, Swamy Ramanand Teerth Marathwada University, Nanded (M.S.), India
3 Dept. of Boitechnology, Indira Gandhi Krishi Vishwavidhyalaya., Raipur, (C.G.), India
     

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To identify putative candidate genes involved in BLB disease resistance, we performed in silico anchoring of the generated QTL genetic marker data to the rice physical map. In total, one QTL responsible for resistance specificity to Xanthomonas oryzae pv. oryzae iso 6 was identified using CT 9993-5-10-1-M / IR 62266-42-6-2 double haploid population. The QTL qBB 10.1, on chromosome 10 RG 4561-EM 14-10 was saturated with 108 markers, 1097 ESTs, and 201 genes. In total, 28 putative positional candidate genes were identified and were classified based on their function. it is suggested that, the generated data can further be used for the dissection and validation of the QTL to understand the molecular mechanisms of the plant pathogen interaction and to develop a resistant variety.

Keywords

QTL, Bacterial Leaf Blight, Expressed Sequence Tags, Candidate Genes, Physical Map.
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  • Identification of Candidate Genes for Bacterial Leaf Blight Resistance in Rice by Integration of Genetic QTL Map with the Physical Map

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Authors

B. P. Niranjan Reddy
National Research Center on Plant Biotechnology, IARI, New Delhi, India
R. K. Deshmukh
Dept. of Boitechnology, Swamy Ramanand Teerth Marathwada University, Nanded (M.S.), India
Bhavna Gupta
Dept. of Boitechnology, Indira Gandhi Krishi Vishwavidhyalaya., Raipur, (C.G.), India
N. K. Deshmukh
Dept. of Boitechnology, Swamy Ramanand Teerth Marathwada University, Nanded (M.S.), India
G. Bhaganagare
Dept. of Boitechnology, Indira Gandhi Krishi Vishwavidhyalaya., Raipur, (C.G.), India
S. M. Shivraj
Dept. of Boitechnology, Indira Gandhi Krishi Vishwavidhyalaya., Raipur, (C.G.), India
T. Singh
Dept. of Boitechnology, Indira Gandhi Krishi Vishwavidhyalaya., Raipur, (C.G.), India
A. S. Kotashane
Dept. of Boitechnology, Indira Gandhi Krishi Vishwavidhyalaya., Raipur, (C.G.), India

Abstract


To identify putative candidate genes involved in BLB disease resistance, we performed in silico anchoring of the generated QTL genetic marker data to the rice physical map. In total, one QTL responsible for resistance specificity to Xanthomonas oryzae pv. oryzae iso 6 was identified using CT 9993-5-10-1-M / IR 62266-42-6-2 double haploid population. The QTL qBB 10.1, on chromosome 10 RG 4561-EM 14-10 was saturated with 108 markers, 1097 ESTs, and 201 genes. In total, 28 putative positional candidate genes were identified and were classified based on their function. it is suggested that, the generated data can further be used for the dissection and validation of the QTL to understand the molecular mechanisms of the plant pathogen interaction and to develop a resistant variety.

Keywords


QTL, Bacterial Leaf Blight, Expressed Sequence Tags, Candidate Genes, Physical Map.