Genetic Diversity Among Aerobic Rice Accessions Using RAPD Markers

Investigation was carried out to evaluate genetic diversity between 103 aerobic rice (Oryza sativa L.) accessions using RAPD markers. Eighty random primers were used to generate the RAPD profiles of 103 aerobic rice accessions. Fourteen markers that showed reproducibility were used to detect polymorphism. Of the 94 bands obtained, 74 (78.72%) were polymorphic. The pair wise similarity ranged from 0.62 to 0.94 with a mean of 0.78. The PIC (polymorphic information content) ranged from 0.01 (OPE7) to 0.36 (OPC14 and OPD9) with an average PIC 0.26. The genotypes were grouped into clusters and sub-clusters. The genotype 109 (DGI 155) was found distinct from all other accessions. A group of RAPD primers showing 100% polymorphism can be used in genetic diversity studies of rice germplasm.