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Genetic Improvement in an Egg Parasitoid T. chilonis for Tolerance to Pesticides


Affiliations
1 Department of Agril. Entomology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), India
     

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Bioassay tests in the laboratory were conducted to study the susceptibility of adult T. chilonis to different concentrations of endosulfan. The newly emerged F1 progeny were subjected to increased concentration of endosulfan. It was found that after the treatment of adult T. chilonis with endosulfan for 15 generations, the LC50 values of endosulfan increased by 13.27% from 0.0437% of base colony (susceptible) to 0.0495% of selected F15 colony (tolerant). To study the mode of inheritance, the programme of back crossing was undertaken. LC50 values of back cross progeny obtained was 0.0476% which was intermediate between susceptible (S) colony (LC50 0.0437%) and F15 selected (R) colony (LC50 0.0495%) indicating that the gene for endosulfan tolerance is completely dominant.

Keywords

Bioassay, Trichogramma, Tolerance, Inheritance, Backcrossing.
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  • Genetic Improvement in an Egg Parasitoid T. chilonis for Tolerance to Pesticides

Abstract Views: 185  |  PDF Views: 0

Authors

M. B. Ingle
Department of Agril. Entomology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), India
S. A. Ghorpade
Department of Agril. Entomology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), India
H. S. Baheti
Department of Agril. Entomology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), India
S. R. Kamdi
Department of Agril. Entomology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), India

Abstract


Bioassay tests in the laboratory were conducted to study the susceptibility of adult T. chilonis to different concentrations of endosulfan. The newly emerged F1 progeny were subjected to increased concentration of endosulfan. It was found that after the treatment of adult T. chilonis with endosulfan for 15 generations, the LC50 values of endosulfan increased by 13.27% from 0.0437% of base colony (susceptible) to 0.0495% of selected F15 colony (tolerant). To study the mode of inheritance, the programme of back crossing was undertaken. LC50 values of back cross progeny obtained was 0.0476% which was intermediate between susceptible (S) colony (LC50 0.0437%) and F15 selected (R) colony (LC50 0.0495%) indicating that the gene for endosulfan tolerance is completely dominant.

Keywords


Bioassay, Trichogramma, Tolerance, Inheritance, Backcrossing.