Asian Journal of Bio Science

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In Vitro Studies on Callus Induction and Plantlet Regeneration in Safflower (Carthamus tinctorius L.)


Affiliations
1 Central Institute for Cotton Research, Post Bag No. 2, Shankarnagar P.O., Nagpur (M.S.), India
2 Green House Laboratory, Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, BARC, Trombay, Mumbai (M.S.), India
3 Department of Agricultural Biotechnology, Marathwada Agricultural University, Parbhani (M.S.), India
     

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Callus induction and regeneration in safflower (Carthamus tinctorius L.) cv. Sharda and PBN-12 was optimized using various explants such as hypocotyls, cotyledonary leaves ischolar_mains and apical shoot buds. The cotyledonary leaves exhibited better response for callus induction as compare to ischolar_mains and hypocotyls on MS supplemented with 5mgl-1 NAA, 0.25mg-1 BAP and 1% sucrose.

Keywords

Callus Induction, Plantlet Regeneration, Safflower.
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  • In Vitro Studies on Callus Induction and Plantlet Regeneration in Safflower (Carthamus tinctorius L.)

Abstract Views: 477  |  PDF Views: 0

Authors

R. L. Chavhan
Central Institute for Cotton Research, Post Bag No. 2, Shankarnagar P.O., Nagpur (M.S.), India
V. Y. Patade
Green House Laboratory, Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, BARC, Trombay, Mumbai (M.S.), India
H. B. Patil
Department of Agricultural Biotechnology, Marathwada Agricultural University, Parbhani (M.S.), India

Abstract


Callus induction and regeneration in safflower (Carthamus tinctorius L.) cv. Sharda and PBN-12 was optimized using various explants such as hypocotyls, cotyledonary leaves ischolar_mains and apical shoot buds. The cotyledonary leaves exhibited better response for callus induction as compare to ischolar_mains and hypocotyls on MS supplemented with 5mgl-1 NAA, 0.25mg-1 BAP and 1% sucrose.

Keywords


Callus Induction, Plantlet Regeneration, Safflower.