Asian Journal of Bio Science

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Effect of Mulberry Nutrition on Protein and Free Amino Acid Levels of Haemolymph of Selected Races of the Silkworm, Bombyx mori L. under Temperate Climates of Kashmir


Affiliations
1 Temperate Sericulture Research Institute Skuast-k, Mirgund, J&K, India
2 Temperate Sericulture Research Institute Skuast-k, Mirgund, J&K
3 Directrate of Extension, Sher-e-kashmir University of Agricultural Sciences and Technology (k), Shalimar, Srinagar, J&K, India
4 Division of Agricultural Statistics, Sher-e-kashmir University of Agricultural Sciences and Technology (k), Shalimar, Srinagar, J&K, India
     

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Changes in the levels of protein and free amino acids in the haemolymph of three selected silkworm breeds viz., SKAU-R-1, SH-6 and NB4D2, fed with selected mulberry varieties like Ichinose (control), Goshoerami (uniformly the best), and Kokuso-20 (least productive) were investigated in 4th moult, and 5th instar larval development during spring and summer seasons. The protein levels in haemolymph were lower at moult in all the three races but the levels showed a sharp increase upon resumption of feeding. The mean levels of protein irrespective of seasons were relatively higher in larvae of SKAU-R-1(44.97 mg/ml) followed by NB4D2 (44.35 mg/ml) and SH6 (42.48 mg/ml). Haemolymph free amino acid levels decreased during 5th instar from the level observed at 4th moult. The mean free amino acid level for the entire 5th instar larval stage in both seasons was relatively higher in NB4D2 (4.55 mg/ml) followed by SKAU-R-1 (4.25 mg/ml) and SH6 (4.17 mg/ml). Free amino acid levels dropped through the 5th instar larval development in all the three races. The changes in the haemolymph protein and free amino acid levels were observed from different base levels in the larvae feeding on different mulberry varieties. Racial differences in the haemolymph protein and free amino acid contents were observed during both seasons in relation to mulberry nutrition being higher in larvae feeding on the foliage of Goshoerami than Ichinose and Kokuso-20.

Keywords

Mulberry, Bombyx Mori, Haemolymph, Protein, Free Amino Acid
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  • Effect of Mulberry Nutrition on Protein and Free Amino Acid Levels of Haemolymph of Selected Races of the Silkworm, Bombyx mori L. under Temperate Climates of Kashmir

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Authors

Awquib Sabhat
Temperate Sericulture Research Institute Skuast-k, Mirgund, J&K, India
M. A. Malik
Temperate Sericulture Research Institute Skuast-k, Mirgund, J&K
A. S. Kamili
Directrate of Extension, Sher-e-kashmir University of Agricultural Sciences and Technology (k), Shalimar, Srinagar, J&K, India
G. N. Malik
Temperate Sericulture Research Institute Skuast-k, Mirgund, J&K, India
S. A. Mir
Division of Agricultural Statistics, Sher-e-kashmir University of Agricultural Sciences and Technology (k), Shalimar, Srinagar, J&K, India
Firdose Ahmad Malik
Temperate Sericulture Research Institute Skuast-k, Mirgund, J&K

Abstract


Changes in the levels of protein and free amino acids in the haemolymph of three selected silkworm breeds viz., SKAU-R-1, SH-6 and NB4D2, fed with selected mulberry varieties like Ichinose (control), Goshoerami (uniformly the best), and Kokuso-20 (least productive) were investigated in 4th moult, and 5th instar larval development during spring and summer seasons. The protein levels in haemolymph were lower at moult in all the three races but the levels showed a sharp increase upon resumption of feeding. The mean levels of protein irrespective of seasons were relatively higher in larvae of SKAU-R-1(44.97 mg/ml) followed by NB4D2 (44.35 mg/ml) and SH6 (42.48 mg/ml). Haemolymph free amino acid levels decreased during 5th instar from the level observed at 4th moult. The mean free amino acid level for the entire 5th instar larval stage in both seasons was relatively higher in NB4D2 (4.55 mg/ml) followed by SKAU-R-1 (4.25 mg/ml) and SH6 (4.17 mg/ml). Free amino acid levels dropped through the 5th instar larval development in all the three races. The changes in the haemolymph protein and free amino acid levels were observed from different base levels in the larvae feeding on different mulberry varieties. Racial differences in the haemolymph protein and free amino acid contents were observed during both seasons in relation to mulberry nutrition being higher in larvae feeding on the foliage of Goshoerami than Ichinose and Kokuso-20.

Keywords


Mulberry, Bombyx Mori, Haemolymph, Protein, Free Amino Acid

References