Asian Journal of Bio Science

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Comparative Analysis of Antibacterial and Antioxidant Activity of Coriandrum sativum, Mentha pipereta and Spinacia oleracea


Affiliations
1 Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agricutlure, Technology and Sciences, Allahabad, U.P., India
     

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The present study was carried out to evaluate the antibacterial and antioxidant activity of the crude ethanolic an acetone extracts of Mentha pipereta, Spinacia oleracea and Coriandrum sativum. The agar well diffusion assay and MIC test were carried out against four strains of bacterial species, viz., Staphylococcus aureus, E. coli, Bacillus pumulis, and Klebsiella pneumonia to determine the sample's antibacterial activity. The extracts of the plants at a concentration of 50 ?l/disc (200 mg/ml) showed minimum to moderate activity against bacteria indicating a broad spectrum activity. Variable concentrations of ethanolic extract of plant samples were effective against various pathogenic bacteria in MIC test. The result indicated the potential usefulness of these plants especially, in treating bacterial infections and justified the need for further investigations and characterization of the bioactive compounds present in the ethanolic and acetone extracts of the plants. On comparing the three plant materials, the chlorophyll content (both Chl A and Chl B) was found to be maximum in Spinacia oleracea leaves. The carotene content was found to be maximum in acetone extract of Mentha pipereta leaves, while minimum in Coriandrum sativum. Recently, attention has focused on phytochemicals as new sources of natural antioxidants. Therefore, the ethanolic crude extracts of the plant samples were screened for total phenols, flavonoids, and free radical scavenging activity. Free radical scavenging activity was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Significant differences in DPPH scavenging activity were found between the species investigated, ranging from 12.71 per cent to 68 per cent. The highest radical scavenging activity was observed in Coriandrum sativum (68% inhibition), followed by Mentha pipereta (61.62%) and Spinacia oleracea (54.72%). The total phenol content of the investigated species ranged from 26 to 75 mg CE/g extract, while flavonoids content ranged from 22 to 24 mg CE/g extract. The findings indicated promising antioxidant activity of crude extracts of the above plants and needs further exploration for their effective use in both modern and traditional system of medicines.

Keywords

Mentha Pipereta, Spinacia Oleracea, Coriandrum Sativum, Staphylococcus Aureus, E. Coli, Bacillus Pumulis, Klebsiella Pneumonie, Flavonoids
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  • Comparative Analysis of Antibacterial and Antioxidant Activity of Coriandrum sativum, Mentha pipereta and Spinacia oleracea

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Authors

Amit Alexander Charan
Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agricutlure, Technology and Sciences, Allahabad, U.P., India
Nidhi Pathak
Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agricutlure, Technology and Sciences, Allahabad, U.P., India
Abhishek Sharan
Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agricutlure, Technology and Sciences, Allahabad, U.P., India

Abstract


The present study was carried out to evaluate the antibacterial and antioxidant activity of the crude ethanolic an acetone extracts of Mentha pipereta, Spinacia oleracea and Coriandrum sativum. The agar well diffusion assay and MIC test were carried out against four strains of bacterial species, viz., Staphylococcus aureus, E. coli, Bacillus pumulis, and Klebsiella pneumonia to determine the sample's antibacterial activity. The extracts of the plants at a concentration of 50 ?l/disc (200 mg/ml) showed minimum to moderate activity against bacteria indicating a broad spectrum activity. Variable concentrations of ethanolic extract of plant samples were effective against various pathogenic bacteria in MIC test. The result indicated the potential usefulness of these plants especially, in treating bacterial infections and justified the need for further investigations and characterization of the bioactive compounds present in the ethanolic and acetone extracts of the plants. On comparing the three plant materials, the chlorophyll content (both Chl A and Chl B) was found to be maximum in Spinacia oleracea leaves. The carotene content was found to be maximum in acetone extract of Mentha pipereta leaves, while minimum in Coriandrum sativum. Recently, attention has focused on phytochemicals as new sources of natural antioxidants. Therefore, the ethanolic crude extracts of the plant samples were screened for total phenols, flavonoids, and free radical scavenging activity. Free radical scavenging activity was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Significant differences in DPPH scavenging activity were found between the species investigated, ranging from 12.71 per cent to 68 per cent. The highest radical scavenging activity was observed in Coriandrum sativum (68% inhibition), followed by Mentha pipereta (61.62%) and Spinacia oleracea (54.72%). The total phenol content of the investigated species ranged from 26 to 75 mg CE/g extract, while flavonoids content ranged from 22 to 24 mg CE/g extract. The findings indicated promising antioxidant activity of crude extracts of the above plants and needs further exploration for their effective use in both modern and traditional system of medicines.

Keywords


Mentha Pipereta, Spinacia Oleracea, Coriandrum Sativum, Staphylococcus Aureus, E. Coli, Bacillus Pumulis, Klebsiella Pneumonie, Flavonoids

References