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In Vitro Antioxidant Activity Studies of Artocarpus gomezianus


Affiliations
1 Dr. P. Sadananda Maiya Centre for Food Science and Research, Benguluru (Karnataka), India
2 Department of Studies and Research in Chemistry, Tumkur University, Tumkur (Karnataka), India

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Extraction of fruits, aerial parts and ischolar_mains from Artocarpus gomezianus were subjected for pharmacognostic analysis. Also these parts were subjected tohydro-alcohol (30:70) extraction by soxhlet extraction technique. The raw materials were dried and powdered and analyzed for various parameters. The moisture content was found to be in the range of 4.97 per cent to 11.09 per cent, extractive values - 14.92 per cent to 18.75 per cent, chloroform solubility 1.15 per cent to 6.99 per cent and water solubility ranged between 4.52 per cent to 5.77 per cent. Fluorescence analysis and ash values were also determined. Extraction yields with 30:70 solvents indicate the quantitative idea about some of the proximate components. The results are important in planning the extraction, phytochemical analysis and determination of their various beneficial biological activities. The study was undertaken to evaluate qualitatively for the contents of carbohydrates, glycosides, saponins, alkaloids, flavonoids, phenolics, tannins, Phytosterols, Triterpenoids, oils and fats present in the extracts from the Fruit, Aerial parts and Roots of Artocarpus gomezianus. The extracts when tested qualitatively for various phytochemicals, they found to contain carbohydrates, Glycosides, alkaloids, tannins, phytosterols and Triterpinoids. However, they do not contain saponins, oils and fats. Quantitative estimation of extracts for total phenols and total flavonoids reveals that most parts contain reasonably higher amounts. The results clearly demonstrate that the extracts can be considered for further studies which evaluate the biological activity such as antioxidant activities. Antioxidant assays such as Nitric oxide scavenging assay, Ferric ion reducing activity assay, ABTS free radical scavenging activity assay and total antioxidant assays were performed to ascertain the potency of the extracts. Fruit extract of Artocarpus gomezianus was found to have maximum ferric ion reducing property than other parts studied. Nitric oxide scavenging activity was found to be higher in fruit followed by aerial and ischolar_main parts. ABTS radical scavenging activity of aerial part extract is found to be 5 mg/ml compared to the standard ascorbic acid with 4 mg/ml. The total antioxidant activity was found to be significantly high for the extract from fruit part than the extracts from aerial and ischolar_main parts. It is clear from the studies that the extracts of aerial parts, fruits and ischolar_mains possess potentially beneficial antioxidant activities. In view of their use in ancient medicine coupled with the recent understandings of these plant species, they may be considered for further exploration as they may yield very potent nutraceuticals.

Keywords

Artocarpus gomezianus, Plant Extracts, Antioxidant, Free Radical Scavenging.
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  • Anonymous (1985). Indian Pharmacopoeia, Vol. II, 3rd Ed., Controller of Publications, Govt. of India, NEW DELHI (INDIA).

  • Balbach, A. and Boarim, D.S.F. (1992). As frutasnamedicina natural. Editoral Missionaria, Sao Paulo.

  • Canter, P.H., Thomas, H. and Ernst, E. (2005). Bringing medicinal plants into cultivation:opportunities and challenges for biotechnology. Trends Biotechnol., 23: 180-185.

  • Das, P., Goswami, K., Chinniah, S., Panda, A., Banerjee, N., Sahu, S. and Achari, N.P.B. (2007). Woodfordiafruticosa: traditional uses and recent findings. J. Ethnopharmacol., 110: 189-199.

  • Favero, J., Corbeau, P., Nicolas, M., Benkirane, M., Trave, G., Dixon, J.F.P., Aucouturier, P., Rasheed, S., Liautard, J.P., Devaux, C. and Dornand, J. (1993). Inhibition of human immunodeficiency virus infection by the lectin Jacalin and by a derived peptide showing a sequence similarity wiyh gp120. European J. Immunol., 23: 179-185.

  • Ferrao, J.E.M. (1999). Fruticultura tropical: especies com frutoscomestiveis, vol. I. Instituto de Investigacao Cientifica Tropical, Lisboa.

  • Finar, I.L. (1959). Organic Chemistry. 2nd Ed., the English language Book society, London (U.K.).

  • Gutierrez, R.M.P., Mitchell, S. and Solis, R.V. (2008). Psidiumguajava: a review of its traditional uses, phytochemistry and pharmacology. J. Ethnopharmacol., 117: 1-27.

  • Hakim, E.H., Achmad, S.A., Juliawaty, L.D., Makmur, L., Syah, Y.M., Aimi, N., Kitajima, M. and Ghisalberti, E.L. (2006). Prenylated flavonoids and related compounds of theIndonesian Artocarpus (Moraceae). J. Natural Medicine, 60: 161-184.

  • Heyne, K. (1987). The Useful Indonesian Plants. Research and Development Agency, The Ministry of Forestry, Jakarta, 659-703 pp.

  • ICUC (2003). International Centre for Underutilized Crops Report, Department of Civiland Environmental Engineering, University of Southhampton, SO 17 1BJ, UK.

  • Jarret, F.M. (1959). Studies in Artocarpus and allied genera. III. A revision of Artocarpussubgenus Artocarpus. J. Arnold Arboretum, 40: 1-298.

  • Kabir, S. (1998). Jacalin: a jackfruit (Artocarpus heterophyllus) seed derived lectin of versatile applications in immunological research. J. Immunological Methods, 212: 193-211.

  • Kokate, C.C., Purohit, A.P. and Gokhale, S.B. (2001). Text Book of Pharmacognosy. Carbohydrate and derived products, drugs containing glycosides, drugs containing tannins, lipids and proteins alkaloids. 7th Ed, Nirali Prekashan, (INDIA).

  • Lansky, E.P., Paavilainen, H.M., Pawlu, A.D. and Newman, R.A. (2008). Ficus spp. (fig): ethnobotany and potential as anticancer and anti-inflammatory agents. J. Ethnopharmacol., 119: 195-213.

  • Le Cointe, P. (1947). Amazonia Brasileira III. Arvires e plantasuteis. Companhiaeditora Nacional, Rio de Janeiro.

  • Middeltone, H. (1956). Systematic Qualitative Analysis. Edward Arnnold Publishers Ltd., London (U.K.).

  • Mojca, S., Petra, K., Majda, H., Andreja, R.H., Marjana, S. and Zeljko, K. (2005). Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem., 89: 191-198.

  • Nenadis, N., Wang, L., Tsimidou, M. and Zhang, H. (2004). Estimation of scavenging activity of phenolic compounds using the ABTS assay. J. Agril. Food Chem., 52: 4669-4674.

  • Oyaizu, M. (1986). Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J. Nutri., 44: 307-315.

  • Peach, T. and Trancey, M.V. (1955). Modern Methods in Plant Analysis. Springer Verlog, Berlin, 387pp.

  • Perry, L.M. (1980). Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses, MIT Press, Cambridge, M.A.

  • Rosenthaler, L. (1930). Chemical investigations of Plants. G. Bell and Sons, London, 23-29: 119-132.

  • Salguero, C.P. (2003). A Thai Herbal Traditional Recipes for Health and Harmony. Findhorn Press, Scotland, 119pp.

  • Shellard, E.J. (1957). Practical Plant Chemistry. Ritman Medical Publishing Co. Ltd., London(U.K.).

  • Spanos, G.A. and Wrolstad, R.E. (1990). Influence of processing and storage on the phenolic composition of Thompson seedless grape juice. J. Agril. & Food Chem., 38 : 1565–1571.

  • Sritularak, B., Tantituvanont, A., Chanvorachote, P., Meksawan, K., Miyamoto, T., Kohno, Y. and Likhitwitayawuid, K. (2010). Flavonoids with free radical scavenging activity and nitric oxide inhibitory effect from the stem bark of Artocarpus gomezianus. J. Medicinal Plants Res., 4 (5): 387-392.

  • Swain, T. and Hillis, W.E. (1959). The phenolic constituents of Prunusdomestica. I. - The quantitative analysis of phenolic constituents. J. Sci. Food Agril., 10 (1): 63-68.

  • Verheij, E.W.M. and Coronel, R.E. (1992). Plant Resources of South-East Asia No. 2. EdibleFruits & Nuts. Prosea, Bogor Indonesia.


Abstract Views: 767




  • In Vitro Antioxidant Activity Studies of Artocarpus gomezianus

Abstract Views: 767  | 

Authors

S. J. Prashanth
Dr. P. Sadananda Maiya Centre for Food Science and Research, Benguluru (Karnataka), India
D. Suresh
Department of Studies and Research in Chemistry, Tumkur University, Tumkur (Karnataka), India
V. H. Potty
Dr. P. Sadananda Maiya Centre for Food Science and Research, Benguluru (Karnataka), India
P. Sadananda Maiya
Dr. P. Sadananda Maiya Centre for Food Science and Research, Benguluru (Karnataka), India

Abstract


Extraction of fruits, aerial parts and ischolar_mains from Artocarpus gomezianus were subjected for pharmacognostic analysis. Also these parts were subjected tohydro-alcohol (30:70) extraction by soxhlet extraction technique. The raw materials were dried and powdered and analyzed for various parameters. The moisture content was found to be in the range of 4.97 per cent to 11.09 per cent, extractive values - 14.92 per cent to 18.75 per cent, chloroform solubility 1.15 per cent to 6.99 per cent and water solubility ranged between 4.52 per cent to 5.77 per cent. Fluorescence analysis and ash values were also determined. Extraction yields with 30:70 solvents indicate the quantitative idea about some of the proximate components. The results are important in planning the extraction, phytochemical analysis and determination of their various beneficial biological activities. The study was undertaken to evaluate qualitatively for the contents of carbohydrates, glycosides, saponins, alkaloids, flavonoids, phenolics, tannins, Phytosterols, Triterpenoids, oils and fats present in the extracts from the Fruit, Aerial parts and Roots of Artocarpus gomezianus. The extracts when tested qualitatively for various phytochemicals, they found to contain carbohydrates, Glycosides, alkaloids, tannins, phytosterols and Triterpinoids. However, they do not contain saponins, oils and fats. Quantitative estimation of extracts for total phenols and total flavonoids reveals that most parts contain reasonably higher amounts. The results clearly demonstrate that the extracts can be considered for further studies which evaluate the biological activity such as antioxidant activities. Antioxidant assays such as Nitric oxide scavenging assay, Ferric ion reducing activity assay, ABTS free radical scavenging activity assay and total antioxidant assays were performed to ascertain the potency of the extracts. Fruit extract of Artocarpus gomezianus was found to have maximum ferric ion reducing property than other parts studied. Nitric oxide scavenging activity was found to be higher in fruit followed by aerial and ischolar_main parts. ABTS radical scavenging activity of aerial part extract is found to be 5 mg/ml compared to the standard ascorbic acid with 4 mg/ml. The total antioxidant activity was found to be significantly high for the extract from fruit part than the extracts from aerial and ischolar_main parts. It is clear from the studies that the extracts of aerial parts, fruits and ischolar_mains possess potentially beneficial antioxidant activities. In view of their use in ancient medicine coupled with the recent understandings of these plant species, they may be considered for further exploration as they may yield very potent nutraceuticals.

Keywords


Artocarpus gomezianus, Plant Extracts, Antioxidant, Free Radical Scavenging.

References