Asian Journal of Bio Science

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Genetic Purity and Diversity in Isoenzyme Peroxidase of Pearl Millet Hybrids [Pennisetum glaucum (L.) Br. R.]


Affiliations
1 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh (Gujarat), India
2 Department of Processing and Food Engineering, College of Agricultural Engineering and Technology, Junagadh Agricultural University, Junagadh (Gujarat), India
     

   Subscribe/Renew Journal


The prospective of Peroxidase Isozyme biochemical marker, have been investigation in the present study, ten pearl millet hybrids [Pennisetum glaucum (L.) R. Br.] Genotypes were used to examine the suitability of peroxidase enzymes for characterization of pearl millet genotypes. Band number 1 (Rm = 0.120) was present in three hybrids i.e. GHB-526, GHB-538 and GHB-719. Band number 2 (0.158) was also present in three hybrids i.e. GHB-538, GHB-577 and GHB-719. Band number 3 (Rm = 0.380) was present in only one hybrid i.e. GHB-719 while band number 4 (Rm = 0.429) was present in five hybrids GHB-538, GHB-719, GHB-732, GHB-235 and GHB-757. All the seven bands were present in only one hybrid GHB-719. 9 DAG stage was found to be more effective as compared to 3 DAG and 6 DAG by showing more number of bands. Among the 10 genotypes studied, two could be differentiated from each other Total 13 bands of peroxidase isozymes were observed at 3, 6 and 9 day after germination (DAG). A total of 15 alleles were generated by isozymes at different DAG (3, 6 and 9 days) with an average of 5 bands per day. Banding pattern at 6 DAG showed that band number1, 2 and 3 (Rm = 0.525, 0.573 and 0.600) were present in all the hybrids except for GHB-744, in band number 1. At 9 DAG seven bands of peroxidase isozymes were observed having Rm value of 0.120, 0.158, 0.380, 0.429, 0.430, 0.478, and 0.760.

Keywords

Genetic Purity, Pearl Millet Hybrids, ADH, GOT.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Dadlani, M. and Varier, A. (1993). Electrophoresis for variety identification. Technical Bulletin. Division of Seed Science and Technology, IARI, New Delhi, pp. 2-5.

  • Eeswara, J.P. and Peiris, B.C.N. (2001). Isoenzyme as marker for identification of mungbean (Vigna radiate (L) Wilcezk). Seed Sci. & Technol., 29 : 249-254.

  • Jaccard, P. (1980). Nouvilles recherchess sur la distribution florale. Bull. Soc. Vaudois Sci. Nat., 44 : 223-270.

  • Lagudah, E.S. and Hanna, W.W. (1989). Species relationship in Pennisetum gene pool : enzyme polymorphism. Theol. Appi.Genet., 78: 801-808.

  • Markert, C.L. and Moller, F. (1959).Multiple forms of enzymes: tissue, on to genetic and species specific patterns. Proc. Natl.Acad. Sci., 45: 753-763

  • Micales, J.A., Bonde, M.R. and Peterson, G.L. (1986). The use of isozyme analysis in fungal taxonomy and genetics.Mycotaxon., 27 : 407-449.

  • Rohlf, F.J. (1998). NTSYS pc. Numerical taxonomy and multivariate analysis system, Verson 2.0, Exeter Software, Setauket, New York.


Abstract Views: 507

PDF Views: 2




  • Genetic Purity and Diversity in Isoenzyme Peroxidase of Pearl Millet Hybrids [Pennisetum glaucum (L.) Br. R.]

Abstract Views: 507  |  PDF Views: 2

Authors

M. B. Vaja
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh (Gujarat), India
P. J. Rathod
Department of Processing and Food Engineering, College of Agricultural Engineering and Technology, Junagadh Agricultural University, Junagadh (Gujarat), India
M. K. Mandavia
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh (Gujarat), India

Abstract


The prospective of Peroxidase Isozyme biochemical marker, have been investigation in the present study, ten pearl millet hybrids [Pennisetum glaucum (L.) R. Br.] Genotypes were used to examine the suitability of peroxidase enzymes for characterization of pearl millet genotypes. Band number 1 (Rm = 0.120) was present in three hybrids i.e. GHB-526, GHB-538 and GHB-719. Band number 2 (0.158) was also present in three hybrids i.e. GHB-538, GHB-577 and GHB-719. Band number 3 (Rm = 0.380) was present in only one hybrid i.e. GHB-719 while band number 4 (Rm = 0.429) was present in five hybrids GHB-538, GHB-719, GHB-732, GHB-235 and GHB-757. All the seven bands were present in only one hybrid GHB-719. 9 DAG stage was found to be more effective as compared to 3 DAG and 6 DAG by showing more number of bands. Among the 10 genotypes studied, two could be differentiated from each other Total 13 bands of peroxidase isozymes were observed at 3, 6 and 9 day after germination (DAG). A total of 15 alleles were generated by isozymes at different DAG (3, 6 and 9 days) with an average of 5 bands per day. Banding pattern at 6 DAG showed that band number1, 2 and 3 (Rm = 0.525, 0.573 and 0.600) were present in all the hybrids except for GHB-744, in band number 1. At 9 DAG seven bands of peroxidase isozymes were observed having Rm value of 0.120, 0.158, 0.380, 0.429, 0.430, 0.478, and 0.760.

Keywords


Genetic Purity, Pearl Millet Hybrids, ADH, GOT.

References