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Method Development and Validation for the Simultaneous Estimation of Aceclofenac, Paracetamol and Chlorzozazone in Pharmaceutical Dosage Forms by High Performance Thin Layer Chromatography
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A simple, rapid, sensitive and highly precise High Performance Thin Layer Chromatographic Method has been developed for the estimation of Paracetamol, Aceclofenac and Chlorzoxazone in tablets. HPTLC was performed on CAMAG LINOMAT IV, TLC Scanner Version 3.20, using toluene, ethyl acetate and glacial acetic acid (17.5:10:0.5 v/v) as mobile phase. The Chromatogram was developed in CAMAG twin trough glass containing mobile phase. The TLC plates were scanned at 271 nm in schimadzu dual wavelength scanner, and Rf value of Paracetamol, Aceclofenac and Chlorzoxazone was found to be 0.12, 0.29 and 0.72, respectively. The linearity of Paracetamol, Aceclofenac and Chlorzoxazone shows a correlation coefficient of 0.9995, 0.9991, and 0.9997, respectively. The proposed method was validated by determining sensitivity, accuracy, precision and system suitability parameters.
Keywords
Paracetamol, Aceclofenac, Chlorzoxazone, Toluene, Ethyl Acetate, Glacial Acetic Acid, HPTLC, Validation.
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