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Determination of Terconazole from Biological Matrix by HPTLC and It’s Characterization by MS
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High Performance Thin layer chromatography tandem mass spectrometry (HPTLC-MS) method was developed and validated for the determination of Terconazole in biological matrix. HPTLC-MS is rapid and economic technique for the appropriate identification and quantitation of active pharmaceutical ingredient in various matrices. Terconazole showed Rf value 0.65±0.018 using Toluene: Ethyl Acetate: Methanol (6:2:2 v/v/v) as mobile phase. Linearity was obtained within concentration range 50-300ng/band with regression coefficient 0.9921. The proposed method showed percentage recovery 99.4±0.8% and % RSD 0.852, verifying that the method is accurate and precise. The recovery after application to biological matrix was 98.1±1.2% proving its applicability to routine use. LOD and LOQ were 11.5 ng/band and 34.9 ng/band respectively. Specificity study showed that the components of biological matrix did not interfere with the analyte, thereby confirming the specificity of the method. Mass spectrometry was resulted in the spectrum showing mass peak at 531.8 m/z. HPTLC-MS method resulted in direct identification and structural characterization of the analytes from the TLC plate which seems to be convenient as well as less time consuming and thus illustrating wide applicability.
Keywords
HPTLC-MS, Mass Spectrometry, Terconazole, Simulated Fluid.
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