Asian Journal of Pharmaceutical Analysis

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Bromatometric Estimation of Cefepime, Cefoperazone, and Cefotriaxone In their Bulk and Dosage Forms


Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
2 Department of Pharmaceutical Chemistry, Al –Azhar University, Assuit, Egypt
     

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Two spectrophotometric methods are described for determination of Cefepime, Cefoperasone andCefotriaxone in bulk and pharmaceutical dosage forms using insitu generated bromine as oxidizing agent and either methylene blue or methyl orange as chromogenic agents. Drugs are treated with known excess of bromine and residual unreacted bromine is determined by treating with fixed amount of either methylene blue or methyl orange then measuring absorbances at 678 nm and 510 nm, respectively. The amount of bromine reacted corresponds to the amount of each drug. Effect of acidity, bromate - bromide volume and reactiontime, on the absorption was studied. Calibration curves were linear over ranges of 1-3 μg.ml-1 for Cefepime,0.4- 1.0 μg.ml-1 for Cefoperazone and 0.3-0.8 μg.ml-1 for Cefotriaxone in case of methylene blue and of 0.05-3.0 μg.ml-1 for Cefepime, 0.75-2.0 μg.ml-1 for Cefoperazone and 0.2-1.4 μg.ml-1 for Cefotriaxone in case of methyl orange. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical forms and results were compared statistically with reference methods.

Keywords

Cefepime, Cefoperazone, Cefotriaxone, Methylene Blue and Methyl Orange.
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  • Bromatometric Estimation of Cefepime, Cefoperazone, and Cefotriaxone In their Bulk and Dosage Forms

Abstract Views: 311  |  PDF Views: 2

Authors

Abdallah A. El-Shanawany
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Sobhy M. El-Adl
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Lobna M. Abdel-Aziz
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Ali F. Hassan
Department of Pharmaceutical Chemistry, Al –Azhar University, Assuit, Egypt

Abstract


Two spectrophotometric methods are described for determination of Cefepime, Cefoperasone andCefotriaxone in bulk and pharmaceutical dosage forms using insitu generated bromine as oxidizing agent and either methylene blue or methyl orange as chromogenic agents. Drugs are treated with known excess of bromine and residual unreacted bromine is determined by treating with fixed amount of either methylene blue or methyl orange then measuring absorbances at 678 nm and 510 nm, respectively. The amount of bromine reacted corresponds to the amount of each drug. Effect of acidity, bromate - bromide volume and reactiontime, on the absorption was studied. Calibration curves were linear over ranges of 1-3 μg.ml-1 for Cefepime,0.4- 1.0 μg.ml-1 for Cefoperazone and 0.3-0.8 μg.ml-1 for Cefotriaxone in case of methylene blue and of 0.05-3.0 μg.ml-1 for Cefepime, 0.75-2.0 μg.ml-1 for Cefoperazone and 0.2-1.4 μg.ml-1 for Cefotriaxone in case of methyl orange. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical forms and results were compared statistically with reference methods.

Keywords


Cefepime, Cefoperazone, Cefotriaxone, Methylene Blue and Methyl Orange.