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HPTLC Method for Routine Quality Control of Ayurvedic Formulation Drakshadi Gutika


Affiliations
1 University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur (C.G.), 492 010, India
2 Chhattisgarh Food and Drug Administration, Raipur (C.G.) 492 001, India
     

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The Drakshadi gutika is effective for amlapitta (hyperacidity), hrddaha (heart disease), kanthadaha (itching of throat), trsna (thirst), murccha (syncope), agnimandaya (digestive impairment), bhrama (vertigo) and amavata (rheumatism) is official in Ayurveduc formulary in India. Quantification of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Ayurvedic medicines/herbal formulations. The Ayurvedic formulation Drakshadi gutika has been prepared as per Ayurvedic formulary of India was estimated HPTLC for its gallic acid content. Three-laboratory batch of Drakshadi gutika were estimated for their gallic acid contents against standard gallic acid solution. The method was validated for linearity, accuracy, limit of detection, limit of quantification, inter-day and intra-day assay precision, repeatability of measurement, and repeatability of sample application. The concentration of gallic acid present in raw material was found to be 8.912±0.41w/w in Terminalia belerica, and 0.73±0.63 w/w in vitis vinifera and in three identical laboratory batch of Drakshadi gutika DG-I, DG-II and DG-III, was found to be 3.307±0.52, 3.301±0.63%, 3.314±0.35 w/w respectively. The gallic acid content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations. Hence the present method is simple, sensitive, precise and accurate and can be adopted for routine quality control of Drakshadi gutika.

Keywords

Gallic acid, Drakshadi gutika, HPTLC, Fingerprinting, Ayurvedic formulation, Quality control parameter.
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  • HPTLC Method for Routine Quality Control of Ayurvedic Formulation Drakshadi Gutika

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Authors

V. Jain
University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur (C.G.), 492 010, India
Tripti Jain
Chhattisgarh Food and Drug Administration, Raipur (C.G.) 492 001, India
Swarnlata Saraf
University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur (C.G.), 492 010, India
S. Saraf
University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur (C.G.), 492 010, India

Abstract


The Drakshadi gutika is effective for amlapitta (hyperacidity), hrddaha (heart disease), kanthadaha (itching of throat), trsna (thirst), murccha (syncope), agnimandaya (digestive impairment), bhrama (vertigo) and amavata (rheumatism) is official in Ayurveduc formulary in India. Quantification of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Ayurvedic medicines/herbal formulations. The Ayurvedic formulation Drakshadi gutika has been prepared as per Ayurvedic formulary of India was estimated HPTLC for its gallic acid content. Three-laboratory batch of Drakshadi gutika were estimated for their gallic acid contents against standard gallic acid solution. The method was validated for linearity, accuracy, limit of detection, limit of quantification, inter-day and intra-day assay precision, repeatability of measurement, and repeatability of sample application. The concentration of gallic acid present in raw material was found to be 8.912±0.41w/w in Terminalia belerica, and 0.73±0.63 w/w in vitis vinifera and in three identical laboratory batch of Drakshadi gutika DG-I, DG-II and DG-III, was found to be 3.307±0.52, 3.301±0.63%, 3.314±0.35 w/w respectively. The gallic acid content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations. Hence the present method is simple, sensitive, precise and accurate and can be adopted for routine quality control of Drakshadi gutika.

Keywords


Gallic acid, Drakshadi gutika, HPTLC, Fingerprinting, Ayurvedic formulation, Quality control parameter.