UV- Spectrophotometric Method Development and Validation for Estimation of Tizanidine and Aceclofenac in Tablet Formulation

The present study deals with UV spectrophotometric method development and validation for estimation of Tizanidine and Aceclofenac tablet dosage form by Viedort's method and first order UV derivative spectrophotometry. The Vierodt's method involves measurement of absorbance at max of Tizaridine and Aceclofenac at 282 nm respectively. The linearity of Tizanidine and Aceclofenac was found to be in the range of 1-10 μg/ml respectively. The % recovery of Tizanidine and Aceclofenac was found out to be 99.2% and 99.69%s respectively. First order CV derivative spectrophotonmetry (D1 method). the zero crossing method was chosen as Tizanidine could he easily analyzed without any interference from Aceclofenac and vice-versa. Tizanidine was determined by measurement of its D1 amplitude at the zero crossing point of Aceclofenac at (270nm), While Aceclofenac was determined by measurement of its D1 amplitude at zero crossing point of Tizanidine at (318 nm) The proposed method was validated as per ICH guidelines.