![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_open_medium.gif)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_restricted_medium.gif)
Validated Non-Extractive Spectrophotometric Methods for Determination of Some Angiotensin II Receptor Antagonists
Subscribe/Renew Journal
Two simple, rapid, sensitive, accurate and non-extractive spectrophotometric methods have been developed for determination of some angiotensin II receptor antagonists (AIIRA's) namely Losartan potassium (Los-K) and Irbesartan in pure forms as well as in their pharmaceutical dosage forms. The methods were based on using chloroformic solutions of either Bromcresol purple (BCP) or Cresol red (CR) for determination of the cited drugs giving highly coloured complexes that measured at 415 nm or 435 nm for both methods respectively. Different variables affecting the reactions were studied and optimized. Under the optimum reactions conditions, linear relationships with good correlation coefficients (0.9960-0.9999) were obtained over the concentration ranges of 2-50 μg/ml. Good accuracy and precision were successfully obtained for the analysis of tablets containing each drug alone or combined with other diuretic drug Hydrochlorothiazide (HCTZ). No interferences could be observed from the coformulated HCTZ as well as the commonly encountered excipeints present in tablets.
Keywords
Spectrophotometry, Angiotensin Ii Receptor Antagonists, Analysis, Losartan Potassium, Irbesartan
Subscription
Login to verify subscription
User
Font Size
Information
- Mycek MJ. Illustrated Reviews Pharmacology., Lippincott’s Williams & Wilkins, Pennsylvania, USA, 2000; 2nd Ed: pp.179- 187.
- Katzung BG. Basic and Clinical Pharmacology. McGraw-Hill, Singapore. 2007; 10th ed: pp.159-177.
- Rao RN, Bompelli S and Maurya PK. High-performance liquid chromatographic determination of anti- hypertensive drugs on dried blood spots using a fluorescence detector – method development and validation. Biomed. Chromatogra. 25; 2011: 1252-1259.
- Pedroso CF, Oliveira JG, Campos FR, Goncalves AG, Trindade ACLB and Pontarolo RA. validated RP–LC method for simultaneous determination of losartan potassium and amlodipine besilate in pharmaceutical preparations. Chromatographia 69; 2009: 201- 206.
- Hafez HM, Abdelaziz LM, Elshanawane AA and Kamal MM. Quantitative determination of four angiotensin-II-receptor antagonists in presence of hydrochlorothiazide by a gradient technique HPLC in their pharmaceutical preparations. Pharm. Anal Acta 3; 2012: 3-7.
- Mhaske RA, Saharsrabudhe S and Mhaske AA. RP-HPLC methods for simultaneous determination of irbesartan, losartan, hydrochlorothiazide and chlrothalidone-application to commercially available drug products. IJPSR. 3; 2012: 1116- 1123.
- Tutunji LF, Tutunji MF, Alzoubi MI, Khabbas MH and Arida AI. Simultaneous determination of irbesartan and hydrochlorothiazide in human plasma using HPLC coupled with tandem mass spectrometry: Application to bioequivalence studies. J. Pharm. Biomed. Anal. 51; 2010: 985-990.
- Zhang M, Wei F, Nie J and Feng YQ. Novel polymer monolith microextraction using a poly (methacrylic acid-ethylene glycol dimethacrylate) monolith and its application to simultaneous analysis of several angiotensin II receptor antagonists in human urine by capillary zone electrophoresis. J. Chromatogr. A 1102; 2006: 294-301.
- Hillaert S and Bossche WV. Optimization and validation of a capillary zone electrophoretic method for the analysis of several angiotensin-II-receptor antagonists. J. Chromatogr. A 979; 2007: 323-333.
- Gonzalez L, Kesolo OA, Jimenez MR and Alonso MR. Application of capillary zone electrophoresis to the screening of some angiotensin II receptor antagonists. Electrophoresis 223; 2002: 223-229.
- Habib IHI, Weshahy SA, Toubar S and El-Alamin MMA. Pharm. Cathodic stripping voltammetric determination of losartan in bulk and pharmaceutical products. Portugaliae Electrochimica Acta 26; 2008: 315-342.
- Rossini PO, Felix FS and Angnes L. A simple and precise conductometric method for the determination of losartan in pharmaceutical products. Central European J. of Chemistry 10; 2012: 1842-1849.
- Cagigal E, Gonzalez L, Alonso RM and Jimenez RM. Experimental design methodologies to optimise the spectrofluorimetric determination of losaratn and valsartan in human urine. J. Pharm. Biomed. Anal. 26; 2001: 477-486.
- El-Shaboury SR, Hussein SA, Mohamed NA and El-Sutohy MM. Spectrofluorimetric method for determination of some angiotensin II receptor antagonists. J. Pharm. Anal. 2; 2012: 12-18.
- Ansari M, Kazemipour M, Khosroui F and Baradaran M. A comparative study first-derivative spectrophotometry and high performance liquid chromatography applied to the determination of losartan potassium in tablets. Chem. Pharm. Bull. 52; 2004: 1166-1170.
- Farouk M, Abd EL-Aziz O, Hemdan A and Shehata M. Simple Novel Spectrophotometric and spectrofluorimetric methods for determination of some antihypertensive drugs. J. of American Science 7; 2011: 300-312.
- Nagavalli D, Vaidhyalingam V, Santha A, Sankar A and Divya O. Simultaneous spectrophotometric determination of losartan potassium, amlodipine besilate and hydrochlorothiazide in pharmaceuticals by chemometric methods. Acta Pharm. 60; 2010: 141–152.
- Darwish IA. Analytical study for the charge transfer complexes of losartan potassium. Anal. Chim. Acta. 549; 2005: 212-220.
- Prabhakar AH and Giridhar R. A rapid colorimetric method for the determination of Losartan potassium in bulk and in synthetic mixture for solid dosage forms. J. Pharm. Biomed. Anal. 27; 2002: 861-866.
- Abdellatef HE. Extractive-spectrophotometric determination of disopyramide and irbesartan in their pharmaceutical formulation. Spectrochimica Acta Part A 66; 2007: 1248-1254.
- Job P. Formation and stability of inorganic complexes in solution. Ann. Chem. 16; 1936: 97.
- Amin AS, Gouda AA, El-Sheikh R. and Zahran F. Spectrophotometric determination of gatifloxacin in pure form and in pharmaceutical formulation, Spectrochimica Acta Part A 67; 2007: 1306-1312.
- Mostafa S, El-Sadek M and Alla EA. Spectrophotometric determination of enrofloxacin and pefloxacin through ion-pair complex formation. J. Pharm. Biomed. Anal. 27; 2008: 173- 180.
- Rahman N, Khan NA and Azmi SNH. Extractive spectrophotometric methods for the determination of nifedipine in pharmaceutical formulations using bromocresol green, bromophenol blue, bromothymol blue and eriochrome black T, Il Farmaco 59; 2004: 47-54.
- Al- Ghannam SM. A simple spectrophotometric method for the determination of -blockers in dosage forms. J. Pharm. Biomed.Anal. 40; 2006: 151-156.
- International Conference on Harmonization, Topic Q1A (R2), available from URL http://www.ich.org/fileadmin/Public_ Web_Site/ICH_Products/Guidelines/Quality/Q1A_R2/Step4/Q1 A_R2__Guideline.pdf
- Deppeler HP and Florey IK. Analytical Profiles of Drug Substances, Academic Press, New York, USA, 1981; chapter 1: pp. 405-441.
- Grimmett MR. Comprehensive Heterocyclic Chemistry. Pergamon Press, Oxford, UK, 1984, 1st Ed: chapter 53, p. 45- 346.
![](https://i-scholar.in/public/site/images/abstractview.png)
Abstract Views: 513
![](https://i-scholar.in/public/site/images/pdfview.png)
PDF Views: 0