Asian Journal of Pharmaceutical Analysis

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

A Simple Ultraviolet Spectrophotometric Method for the Estimation of Docetaxel in Bulk Drug and Formulation


Affiliations
1 Department of Pharmacy, Shri Jagdish Prasad Jhabarmal Tibrewala University, Vidyanagari, Jhunjhunu, Rajasthan, India
     

   Subscribe/Renew Journal


The present investigation aimed at development and validation of an analytical method for the estimation of Docetaxel based on Ultra-violet Spectrophotometry. The reported methods of analysis include the chromatographic methods such as HPLC (1-2), RP-HPLC (3-4), LC-MS/MS (18) and HPLC/MS in plasma (5-7). An ion-pair extractive spectrophotometric method has been reported in the literature for the determination of a docetaxel (8). Chromatographic methods are complex, tedious, time consuming and are not suitable for routine analysis. However, Ultraviolet spectrophotometric methods which are simple and fast are ideal for routine analysis. Docetaxel exhibits a sharp peak at 229nm when scanned in the Ultraviolet region. Thus, it was selected as the analytical wave-length. The developed method was found to be simple, sensitive, accurate, precise and specific as evidenced by non-interference from the excipients present in the marketed injection. Linearity was observed in the range of 5μg/ml to 45μg/ml and Accuracy was found between 99.36 to 101.75%. The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.174μ/ml&0.194μ/ml respectively.

Keywords

Docetaxel, Validation, UV Spectroscopic Method
Subscription Login to verify subscription
User
Notifications
Font Size


  • Harry Gouvas: Use of Sodium Hyaluronate in the treatment of Osteoarthritis, Greece, 2011Dilip kumar Renapurkar, Shobana Mathur and K. Jagdishwer rao,Evaluation of efficacy and safety of diacerein in osteoarthritis of knee joint, Jul-Sep.2010,Vol.1(3)

  • Han-Chun Yao, Xiao-Feng Yang, Sensitive Determination of Nanogram Levels of Diacerein in a Pharmaceutical Formulation by Flow Injection Chemiluminescence Analysis, Journal of the Chinese Chemical Society, 2007, 54, 949-956

  • http://en.wikipedia.org/wiki/Diacerin

  • G.Abirami, K.Anandakumar, R.Velmurugan, Development And Validation of UV-Spectroscopy Method For The Determination of Diacerin Hydrochloride in Pharmaceutical Formulation, Journal of Pharmacy Research,2012;5(4),1949-1951.

  • D M Dhaduk, B G Rathod, P B Patel, Estimation of Sildenafil Citrate and Diacerin Hydrochloride in their Combined Dosage Form by Validated UV Spectroscopic method, Inventi Rapid: Pharm Analysis & Quality Assurance , Vol. 2012 , Article ID- " Inventi:ppaqa/337/12 "

  • P.Selvam, U.Mahalingam, D.Sridharan, and A.Thenmozhi. A Simple UV Spectrophotometric Determination of Diacerein In Pure and In Pharmaceutical Dosage Form, Asian Journal of Biochemical and Pharmaceutical Research, Vol. 1,( 3) 2011

  • Sandeepa Bhoir, Shashikant Dhole, Nilesh Kulkarni, Prajakta Sangole, Sonal Thorat, Dhanashree Bhoite, Novel and validated spectrophotometric estimation of diacerein in bulk and capsule formulation using mixed hydrotropic solubilisation approach, International Journal of Pharmacy and Pharmaceutical Sciences, Vol 4, Suppl 4, 2012

  • Sohan S. Chitlange, Ganesh R. Pawbake, Amir I. Mulla, Sagar B. Wankhede, Simultaneous Spectrophotometric Estimation of Diacerein and Aceclofenac in tablet dosage form, Der Pharma Chemica, 2010, 2(1): 335-341.

  • Krishna R Gupta, Varun E Samrit, Vivek S Thakur and A. T. Hemke, UV-Spectrophotometric estimation of Diacerein in pharmaceutical formulation, Journal of Chemical and Pharmaceutical Research, 2010, 2(3):467-472

  • Narendra Kumar Nyola and Naresh Kalra, Spectrophotometric determination of Diacerin in bulk and pharmaceutical formulation, International Journal of Pharma and Bio Sciences, Oct-Dec.2010,Vol.1(4)

  • Ravindra Pandey,P.O.Patil,M.U.Patil,P.K.Deshmukh,S.B.Bari, Quantitative estimation of Diacerein in bulk and capsule formulation using mixed hydrotropic solubilisation by UV spectroscopy and first order derivative method using area under curve method,Pharmaceutical Methods,Jan-June 2012,Vol 3 (1),4-8

  • K.R .Sreejith,.K.Premalatha, Novel Spectrophotometric Methods for Estimation of Diacerein from Formulations, International Journal of Research in Pharmaceutical and Biomedical Sciences, Vol. 2 (3) Jul – Sep 2011,992-999

  • R Sivakumar, PK Nallasivan, KC Saranya, WD Sam Solomon, T Akelesh, R Venkatnarayanan Visible spectrophotometric estimation of diacerein in bulk and pharmaceutical dosage forms, Pharm Analysis, Year : 2010 ,Volume : 2 ,(4) Page : 414-41

  • R Sivakumar, PK Nallasivan, KC Saranya, Solomon WD Sam, T Akelesh, and R Venkatnarayanan, Visible Spectrophotometric Estimation of Diacerein in Bulk and Pharmaceutical Dosage Forms, J Young Pharm. 2010 Oct-Dec; 2(4): 414–416

  • Hamilton CL, Cornpropst JD., Determination of Diacerin, an investigational agent with the potential for treating depression, and its mono- and di-desmethyl metabolites in human plasma using column-switching high-performance liquid chromatography, J Chromatogr.,1993;612(2):253-61.

  • Abha D. Giri, Vidhya K. Bhusari, Sunil R Dhaneshwar, Validated HPLC Method for Simultaneous Quantitation of Tadalafil and Diacerin Hydrochloride in Bulk Drug and Formulation,, International Journal of Pharmacy and Pharmaceutical Sciences,2012;4(2):654-658.

  • S. P. Gandhi, M. G. Dewani, T. C. Borole and M. C. Damle, Development and validation of stability Indicating HPLC method for determination of Diacerein and aceclofenac as bulk drug and In tablet dosage form, International journal of research in pharmacy and chemistry, 2011, 1(4)

  • Keddal G. Lalithaa, Thangavel Venkatachalamb, Ramaraj Srinivasanb, Ponnusamy Kalaiselvib, Nagappan Kannappan, A Simple HPLC Method for Quantitation of Diacerein in Tablet Dosage Form, Eurasian J. Anal. Chem. 5(1): 81-88, 2010

  • Krishna R Gupta, Varun E. Samrit, Anuradha H Mahapatra, Sudhir G.Wadodkar, Stability indicating HPLC assay method for Diacerein and Aceclofenac in tablets, 2011Volume 1, (3)

  • Rao J, Chauhan K, Mahadik KR, Kadam SS. A stabilityindicating high performance liquid chromatographic method for the determination of diacerein in capsules. Indian J Pharm Sci 2009;71:24-9

  • S. P. Gandhi, M. G. Dewani, T. C. Borole and M. C. Damle, Development and validation of stability indicating HPLC method for determination of Diacerein AND Aceclofenac as bulk drug and in tablet dosage form, international journal of research in pharmacy and chemistry, 2011, 1(4),779-806

  • Keddal G. Lalithaa, Thangavel Venkatachalamb, Ramaraj Srinivasanb, Ponnusamy Kalaiselvib, Nagappan Kannappanc, A Simple HPLC Method for Quantitation of Diacerein in Tablet Dosage Form, Eurasian J. Anal. Chem. 5(1): 81-88, 2010

  • KS Rao, NK Keshar, J Datta, Stability indicating RP-HPLC method for the simultaneous estimation of Diacerein and Aceclofenac in bulk and pharmaceutical dosage forms Inventi Impact: Pharm Analysis & Quality Assurance , Vol. 2010

  • D. Kiran Kumar, K.S. Nataraj, K. Kesinath Reddy, S. Suresh Kumar, Method Development and Validation for analysis of Diacerin in Bulk and Pharmaceutical Dosage Form by RP-HPLC.

  • N. Kannappan, Madhukar A., R. Srinivasan, R.L.A. Srinivas , C H. Naveen Kumar, Mannavalan. R, Analytical method development and validation of Diacerein Tablets by RP-HPLC, Vol.2, No.1, , Jan-Mar 2010, 143-148.

  • Narendra Nyola, Govinda Samy Jeyabalan, N Kalra, Gazala Parveen, and Suresh Choudhary, Development and Validation of a RP-HPLC Method for Simultaneous Estimation of Diacerein and Aceclofenac in Pharmaceutical Dosage Form, Research and Reviews: Journal of Pharmaceutical Analysis, Oct–Dec, 2012,Vol 1 ( 1 )

  • Arun Shirwaikar, A. Sarala Devi, K Premalatha and KR. Sreejith, Determination of Diacerein in Rabbit Plasma by Liquid Chromatography - Mass Spectroscopy and Its Application to Bioavailability Study, International Journal of Research in Pharmaceutical and Biomedical Sciences, Oct – Dec 2012,Vol. 3 (4),1738-1744

  • S. P. Gandhi, M. G. Dewani, T. C. Borole, and M. C. Damle,Development and Validation of Stability Indicating HPTLC Method for Determination of Diacerein and Aceclofenac as Bulk Drug and in Tablet Dosage Form, E-Journal of Chemistry, 2012, 9(4), 2023-2028


Abstract Views: 1220

PDF Views: 1




  • A Simple Ultraviolet Spectrophotometric Method for the Estimation of Docetaxel in Bulk Drug and Formulation

Abstract Views: 1220  |  PDF Views: 1

Authors

Mahawar Sheetal
Department of Pharmacy, Shri Jagdish Prasad Jhabarmal Tibrewala University, Vidyanagari, Jhunjhunu, Rajasthan, India

Abstract


The present investigation aimed at development and validation of an analytical method for the estimation of Docetaxel based on Ultra-violet Spectrophotometry. The reported methods of analysis include the chromatographic methods such as HPLC (1-2), RP-HPLC (3-4), LC-MS/MS (18) and HPLC/MS in plasma (5-7). An ion-pair extractive spectrophotometric method has been reported in the literature for the determination of a docetaxel (8). Chromatographic methods are complex, tedious, time consuming and are not suitable for routine analysis. However, Ultraviolet spectrophotometric methods which are simple and fast are ideal for routine analysis. Docetaxel exhibits a sharp peak at 229nm when scanned in the Ultraviolet region. Thus, it was selected as the analytical wave-length. The developed method was found to be simple, sensitive, accurate, precise and specific as evidenced by non-interference from the excipients present in the marketed injection. Linearity was observed in the range of 5μg/ml to 45μg/ml and Accuracy was found between 99.36 to 101.75%. The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.174μ/ml&0.194μ/ml respectively.

Keywords


Docetaxel, Validation, UV Spectroscopic Method

References