![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_open_medium.gif)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_restricted_medium.gif)
Development and Validation of UV Spectrophotometric Method for the Quantitative Estimation of Eugenol
Subscribe/Renew Journal
Eugenol is an allyl chain-substituted guaiacol and finds variety of applications. Thus development of a validated UV spectrophotometric method will always be advantageous as the method is simple and rapid. The method was validated according to International Conference on Harmonisation (ICH) guidelines Q2(R1) with respect to linearity and range, precision, accuracy, detection limit (DL) and quantitation limit (QL). The detection limit (DL) and quantitation limit (QL) were determined as per the ICH guidelines and were found to be 0.82 and 2.48 μg mL-1 respectively. The method is expected to be useful in a variety of industries where eugenol finds its application.
Keywords
ICH, Linearity, Range, Precision, Accuracy, Detection Limit, Quantitation Limit
Subscription
Login to verify subscription
User
Font Size
Information
- Pramod K, Ansari SH and Ali J. Eugenol: A natural compound with versatile pharmacological actions. Nat Prod Commun. 5 (12); 2010: 1999-2006.
- Kriegel C, Kit KM, McClements DJ, Weiss J. Nanofibers as carrier systems for antimicrobial microemulsions. II. Release characteristics and antimicrobial activity. J Appl Polym Sci. 118; 2010: 2859–2868.
- Pokharkar VB, Shekhawat PB, Dhapte VV, Mandpe LP. Development and optimization of eugenol loaded nanostructured lipid carriers for periodontal delivery. Int J Pharm Pharm Sci. 3; 2011: 138-143.
- Gomes C, Moreira RG, Castell-Perez E. Poly (DL-lactide-coglycolide) (PLGA) nanoparticles with entrapped transcinnamaldehyde and eugenol for antimicrobial delivery applications. J Food Sci. 76 (2); 2011: N16-N24.
- Shu-Ya J. 2010. Encapsulation of active substance in nanocapsules by emulsion-diffusion method [Accessed 12 February 2011]. Available from: URL: http://140.121.155.217/ seminar/19932033-1.pdf.
- Shah A, Garg A, Sairam K, Singh S. 2010. Pharmacological evaluation of eugenol loaded solid lipid nanoparticles in irritable bowel syndrome [Accessed 10 March 2011]. Available from: URL: www.scientificipca.org/paper/2009/09/25/20090925 1910570A.doc.
- Chen F, Shi Z, Neoh KG, Kang ET. Antioxidant and antibacterial activities of eugenol and carvacrol-grafted chitosan nanoparticles. Biotechnol Bioeng. 104 (1); 2009: 30-39.
- Jadhav BK, Khandelwal KR, Ketkar AR, Pisal SS. Formulation and evaluation of mucoadhesive nanocapsules containing eugenol for the treatment of periodontal diseases. Drug Dev. Ind. Pharm. 30; 2004: 195-203.
- Chaieb K, Hajlaoui H, Zmantar T, Kahla-Nakbi AB, Rouabhia M, Mahdouani K, Bakhrouf A. The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): a short review. Phytother. Res. 21; 2007: 501-506.
- ICH-Guidelines Q2(R1). Validation of Analytical Procedures: Text and Methodology. Geneva, Switzerland: 2005.
![](https://i-scholar.in/public/site/images/abstractview.png)
Abstract Views: 557
![](https://i-scholar.in/public/site/images/pdfview.png)
PDF Views: 0