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Venkateswara Rao, B.
- Simultaneous UV Spectrophotometric Method for the Determination of Tenofovir, Efavirenz and Lamivudine in Bulk and Combined Dosage Form
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Authors
Affiliations
1 Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram, Guntur – 19, Andhra Pradesh, IN
1 Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram, Guntur – 19, Andhra Pradesh, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 6, No 4 (2016), Pagination: 253-258Abstract
The aim of the present work is to develop a simple, accurate, precise and reproducible UV spectrophotometric method for the simultaneous determination of Tenofovir, Efavirenz and Lamivudine in bulk and pharmaceutical dosage form. The stock solutions of the standard drugs were prepared in methanol followed by the further required dilutions with distilled water. This method involves the formation and solving of simultaneous equations at 260nm, 347nm and 272 nm, as absorbance maxima of Tenofovir, Efavirenz and Lamivudine respectively. Beer's law obeyed the concentration range of 10 - 40mcg/mL, 5 - 20 mcg/mL and 5 - 20 mcg/mL for Tenofovir, Efavirenz and Lamivudine respectively. The results of analysis were validated statistically and by recovery studies. The % RSD for the recovery study was less than 2. The proposed method can be effectively used for the simultaneous estimation of these three drugs in bulk and combined tablet dosage form.Keywords
Tenofovir, Efavirenz and Lamivudine, Simultaneous Equation Method, Method Validation.
- A Novel RP-HPLC Method Development and Validation for the Determination of Pioglitazone and Glimepiride in Bulk and Pharmaceutical Formulations
Abstract Views :311 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmaceutical Analysis, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram, Guntur, Andhra Pradesh, IN
1 Department of Pharmaceutical Analysis, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram, Guntur, Andhra Pradesh, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 7, No 3 (2017), Pagination: 145-150Abstract
A simple, precise, accurate, reproducible and economical stability- indicating reverse phase liquid chromatography method was developed and validated for the quantitative simultaneous estimation of Pioglitazone and Glimepiride in marketed formulations. Estimation of drugs in this combination was done with a C18 column (Kromasil 100-5 column, 250mm x 4.6mm) using mobile phase of composition phosphate buffer pH 7: Acetonitrile in ratio 60:40 v/v. The flow rate was 0.8 ml/min and the effluents were monitored at 230 nm. The retention time of Pioglitazone and Glimepiride were 6.71 min and 8.38 min respectively. The method was found to be linear over a range of 10-50 μg/ml for Pioglitazone and 3-15 μg/ml for Glimepiride. The established method proved as reproducible one with a % RSD value of less than 2 and having the robustness and accuracy within the specified limits. Assay of marketed formulation was determined and find with 96% and 98% for Pioglitazone and Glimepiride respectively. The method was validated according to the guidelines of International Council for Harmonization (ICH) and was successfully employed in the estimation of commercial formulations. This liquid chromatographic method can be applied for the qualitative and quantitative determination of selected drugs by the modern chemist.Keywords
Pioglitazone, Glimepiride, RP-HPLC and Method validation.References
- Martindale (The complete drug reference) 2005, 34th edition, 632 and 655.
- Merck Index 14th edition, 2006, 9151 and 3568.
- Indian Pharmacopeia 2014, 7th edition, Vol 2 and 3, 1652 and 2834.
- K. S. Lakshmi T. Rajesh. Development and Validation of RPHPLC Method for Simultaneous Determination of Glipizide, Rosiglitazone, Pioglitazone, Glibenclamide and Glimepiride in pharmaceutical dosage forms and human plasma. Journal of the Iranian Chemical Society. 8(1); 2011: 31-37.
- Sami El Deeb, Udo Schepers, Hermann Watzig. Fast HPLC Method for the Determination of Glimepiride, Glibenclamide, and Related Substances using Monolithic Column and Flow Program.Journal of Separation Science. 29(11); 2006: 1571-1577.
- P. K. Sahoo, R. Sharma, and S. C. Chaturvedi. Simultaneous Estimation of Metformin Hydrochloride and Pioglitazone Hydrochloride by RPHPLC Method from Combined Tablet Dosage Form. Indian Journal of Pharmaceutical Sciences. 70(3); 2008: 383–386.
- Karthik Arumugam, Subramanian Ganesan, Chamallapudi Mallikharjuna Rao, Nayanabhirama Udupa. Simultaneous Determination of Pioglitazone and Glimepiride in Bulk Drug and Pharmaceutical Dosage Form by RP-HPLC Method. Pakistan Journal of Pharmaceutical Sciences. 21(4); 2008: 421-425.
- Gadapa Nirupa, and Upendra M. Tripathi. RP-HPLC Analytical Method Development and Validation for Simultaneous Estimation of Three Drugs: Glimepiride, Pioglitazone, and Metformin and its Pharmaceutical Dosage Forms. Journal of Chemistry. 2013: 1-8.
- D. Adukondalu, P. S. Malathy, J. V. Rao, and Y. M. Rao, Development and Validation of HPLC Method for Detection of Pioglitazone hydrochloride in Dosage Forms. International Journal of Pharmacy and Biological Sciences. 1(4); 2011: 474–478.
- D. B. Pathare, A. S. Jadhav, and M. S. Shingare, RP-LC Determination of the cis-isomer of Glimepiride in a Bulk Drug Substance. Chromatographia. 66(7-8); 2007: 639–641.
- N. Satheeshkumar , S. Shantikumar, R. Srinivas. Pioglitazone: A review of analytical methods. Journal of Pharmaceutical Analysis. 4(5); 2014: 295–302.
- Deepti Jain, Surendra Jain, Deepak Jain, Maulik Amin. Simultaneous Estimation of Metformin Hydrochloride, Pioglitazone Hydrochloride, and Glimepiride by RP-HPLC in Tablet Formulation. Journal of Chromatographic Science. 46(6); 2008: 501-504.
- G. Navaneethan, K. Karunakaran and K. P. Elango. Simultaneous Estimation of Pioglitazone, Glimepiride and Glimepiride Impurities in Combination Drug Product by a Validated StabilityIndicating RP-HPLC Method. Journal of Chilean of Chemical Society. 56(3); (2011): 815-818.
- M Suchitra, D Sunitha, C Parthiban, B Siddartha and C Madhavi. Method Development and Validation of Metforminm, Glimepiride and Pioglitazone in Tablet Dosage Forms by RP-HPLC. International Research Journal of Pharmacy. 4(8); 2013: 250-254.
- Rani G Shobha, Lohitha M, Preethi P Jaya, Madhavi R, Sunisitha B and Mounika D. Glimepiride Review of Analytical Methods. Asian Journal of Pharmaceutical Analysis. 4(4); 2014: 178-182.
- Khohinur Hossain , Asma Rahman , Md. Zakir Sultan , Farhana Islam , Md. Akteruzzaman , Md. Abdus Salam and Mohammad A. Rashid. A Validated RP-HPLC Method for Simultaneous Estimation of Antidiabetic Drugs Pioglitazone HCl and Glimepiride. Bangladesh Pharmaceutical Journal. 16(1); 2013: 6975.
- M. S. V. Sakuntala, S. V. U. M. Prasad, S. Sri Devi, S. Kishore Yadav and K. Srinivas Reddy. A RP- HPLC Method Development and Validation for the Simultaneous Estimation of Glimepiride and Pioglitazone HCl in Tablet Dosage Forms. Journal of Chemical and Pharmaceutical Research. 4(1): 2012:154-159.
- Ravi Sharma, Gagan Sharma and Darpan chopra, Analytical Method Development and Validation for the Simultaneous Estimation of Glimepiride and Pioglitazone in Tablet Dosage Form by RP-HPLC. International Journal of Pharmaceutical Sciences and Research. 2(3); 2012: 637-641.
- Kishore L, Kaur N. Estimation of Pioglitazone and Glimepiride in its Pharmaceutical Dosage Form by Spectrophotometric Methods. Der Pharmacia Lettre 4; 2011:276-284.
- Freddy HH, Dharmendra LV. Simultaneous Estimation of Glimepiride, Rosiglitazone and Pioglitazone Hydrochloride in the Pharmaceutical Dosage Form. E-Journal of Chemistry. 7(4); 2010:1326-33.
- Praveen Kumar Reddy B, Boopathy D, Bibin Mathew, Prakash M, Perumal P. Method Development and Validation of Simultaneous Determination of Pioglitazone and Glimepiride in Pharmaceutical Dosage Form by RP-HPLC. International Journal of Chem Tech Research. 2(1); 2010: 50-53.
- Kalyankar. T.M, Badguja. M.R, Mitkare S.S and Kakde R.B. HPTLC Method for Simultaneous Analysis of Pioglitazone HCL and Glimepiride in Pharmaceutical Preparations. Journal of Pharmacy Research. 3(12); 2010: 3118-3120.