Author Details

A reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the estimation of Dexibuprofen in bulk drug and pharmaceutical dosage forms. The quantification was carried out on Waters Symmetry C18 column in isocratic mode, with mobile phase consisting of Acetonitrile and water in the ratio of 55:45%(v/v), adjusted to pH 2.5 with orthophosphoric acid. The mobile phase was pumped at a rate of 1.5mL/min and detection was carried out at 214nm and linearity was found to be in the range of 40 to 160μg/mL. The correlation co-efficient was found to be 0.9991. The % recovery values were found to be in the range of 99.0 to 102%. Validation of the proposed method has also been done.

Keywords

Dexibuprofen, Isocratic, RP-HPLC, Estimation, Tablets.

Full Text

Development and Validation of UV Spectrophotometric Method of Darifenacin Hydrobromide in Bulk and Tablet Dosage Form

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Authors

D. Sridharan ¹, Umarani A. Thenmozhi ¹, L. Pavan Kumar ¹, Aswani Dutt Chintalapati ¹, M. Venkata Ramanaiah ¹, Yelika Phanikishore ¹

Affiliations
1 Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil-626 126, Tamilnadu, IN

Source

Asian Journal of Pharmaceutical Analysis, Vol 1, No 3 (2011), Pagination: 43-45

Abstract

A simple, accurate and specific UV spectrophotometric method was developed and validated for the estimation of Darifenacin hydrobromide in bulk and tablet dosage form. Analyte showed the absorption maxima in 0.1M Hydrochloric acid at 284nm. The percentage recovery of Darifenacin Hydrobromide was 99.69±0.14%. The developed method was validated with respect to linearity, accuracy, precision and specificity. Beer's law was obeyed in the concentration range of 20.00μg/mL to 46.50μg/mL, having line equation y=0.059x+0.0177 with correlation coefficient of 0.999. Results of the analysis were validated statistically as per ICH guidelines.

Keywords

UV Spectrophotometry, Darifenacin, Hydrobromide, Estimation, Tablets.

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