Asian Journal of Pharmaceutical Analysis

Gandla Kumaraswamy ¹, Gandla Lalitha ¹, N. Ravindra ¹, K. B. Pradeesha ¹

Affiliations
1 Department of Pharmaceutical Analysis, Chilkur Balaji College of Pharmacy, Aziz Nagar –Hyderabad, 500075, Telangana, IN

Abstract

A simple, accurate, rapid and precise isocratic reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous determination of Amlodipine, Atenolol and Hydrochlorothiazide in capsules. The chromatographic separation was carried out on an cosmosil packed column 5C18-MS-I I analytical column (250×4.6 mm; 5 μm) with a mixture of Phosphate buffer:Acetonitrile:Methanol pH 6 adjusted with Ortho phosphoric acid (30:20:50, v/v) as mobile phase; at a flow rate of 1 ml/min. UV detection was performed at 240nm. The retention times were 2.637, 3.148 and 8.492min. for Hydrochlorothiazide, Atenolol and Amlodipine respectively. Calibration plots e linear (r2>0.998) over the concentration range 2-12μg/ml for Amlodipine , 10-60μg/ml Atenolol and 2-12μg/ml for Hydrochlorothiazide. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. The proposed method was successfully used for quantitative analysis of capsules. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of Hydrochlorothiazide, Amlodipine and Atenolol in bulk drug and capsule dosage form.

Keywords

Hydrochlorothiazide, Amlodipine, Atenolol, and Tablet Dosage Forms, RP-HPLC.

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Gandla Kumaraswamy ¹, N. Ravindra ¹, B. Jyothsna ¹

Affiliations
1 Department of Pharmaceutical Analysis, Chilkur Balaji College of Pharmacy, Aziz Nagar-Hyderabad, 500075 Telangana, IN

Abstract

A simple, accurate, rapid and precise isocratic reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous determination of Aspirin, Amlodipine and Atenolol in capsules. The chromatographic separation was carried out on an Cosmosil packed C18-MS-II(4.6 I.D.x250mm;5μ particle size) with a mixture of acetonitrile: ammonium acetate buffer pH 4.6 adjusted with 30%v/v acetic acid (80:20, v/v) as mobile phase; at a flow rate of 0.8 ml/min. UV detection was performed at 233 nm. The retention times were 4.415, 3.688 and 3.001 min. for Aspirin, Amlodipine and Atenolol, respectively. Calibration plots were linear (r²>0.998) over the concentration range 1-6 μg/ml for Amlodipine , 5-30 μg/ml for Aspirin and 2.5-15 μg/ml Atenolol. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. The proposed method was successfully used for quantitative analysis of capsules. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of Aspirin, Amlodipine and Atenolol in bulk drug and capsule dosage form.

Keywords

Aspirin, Amlodipine, Atenolol, RP-HPLC, Tablet Dosage Forms.

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