Asian Journal of Pharmaceutical Analysis

A. Aboul-Kheir ¹, Hanaa Saleh ¹, Magda M. El-Henawee ¹, M. N. Sharf El-Din ¹

Affiliations
1 Analytical Chemistry Department, Zagazig University, Zagazig, EG

Abstract

Simple and accurate spectrophotometric methods for determination of Lisinopril and Cefixime in bulk and dosage forms are described. The First method is used for determination of Lisinopril through ternary complex formation between Lisinopril, Congo Red and either Nickel or Copper in presence of borate buffer PH 7.5, colour produced has peak maxima at 499 and 497, respectively. Beer's law is obeyed in range of 20-80 μg and 10-50 μg, respectively. Second method is used for estimation of Cefixime through formation of ion pair complex with Congo Red in presence of borate buffer PH 7.5, produced complex has a peak maximum at 595 nm. Beer's law is obeyed in range of 20-65 μg. Limit of detection and quantitation and Sandel sensitivity has been calculated. Proposed methods were applied for determination of both drugs in some of their pharmaceutical preparations. The Proposed methods were validated and obtained results were compared statistically with reference methods.

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A. Aboul-Kheir ¹, Hanaa Saleh ¹, Magda M. El-Henawee ¹, M. N. Sharf El-Din ¹

Affiliations
1 Analytical Chemistry Department, Zagazig University, Zagazig, EG

Abstract

Two spectrophotometric methods are described for determination of Lamotrigine, Minoxidil and Cefixime in bulk and pharmaceutical dosage forms using insitu generated bromine as oxidizing agent and either methyl orange or methylene blue as chromogenic agents. Drugs are treated with known excess of bromine and residual unreacted bromine is determined by treating with fixed amount of either methyl orange and measuring absorbance at 508 nm or methylene blue and measuring absorbance at 667 nm for minoxidil and lamotrigine and at 747 nm for cefixime. The amount of bromine reacted corresponds to the amount of each drug. Calibration curves were linear over ranges of 4.0 - 30.0, 0.5 - 4.0 and 0.5 - 5.0 μg.ml^-1 for lamotrigine, minoxidil and cefixime, respectively in case of methyl orange and of 5.0 - 40.0, 0.5 - 7.0 and 2.0 - 5.5 μg.ml^-1 for lamotrigine, minoxidil and cefixime, respectively in case of methylene blue. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical forms and results were compared statistically with reference methods.

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