Asian Journal of Pharmaceutical Analysis

Vedantika Das ¹, Ashwini Sambherao ¹, Saroj Gajare ¹, Amar Zalte ¹, R. B. Saudagar ¹

Affiliations
1 Department of Quality Assurance, Department of Pharmaceutical Chemistry, R.G. Sapkal College of Pharmacy, Anjaneri, Nasik, IN

Abstract

A simple, accurate, precise, and sensitive a highly selective ultra violet spectrophotometric method has been developed for the validation and estimation in bulk and dosage form. It shows maximum absorbance 286 nm. Beer-Lambert's was obeyed in the concentration range of in the range of 15-55μg/ml. The method was successfully validated in accordance to ICH guideline for accuracy, precision, linearity and robustness. The linear regression analysis data for calibration plots showed good linear relationship and obtain correlation factor 0.9927 for Drotaverine. Thus method was successfully applied for routine analysis of Drotaverine in bulk samples.

Keywords

Drotaverine, ICH, UV- Spectroscopy, Accuracy, Precision.

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Ashok P. Pingale ¹, A. R. Rote ², R. B. Saudagar ³, Rima R. Patil ², G. B. Jadhav ³

Affiliations
1 Department of Pharmaceutical Chemistry, MVP College of Pharmacy, University of Pune, Maharashtra, Nashik 422002, IN
2 Department of Pharmaceutical Chemistry, M.G.V’s Pharmacy College (Pune University), Mumbai, IN
3 KCT’s R. G. Sapkal College of Pharmacy, Anjineri, Nashik, Maharashtra, IN

Abstract

Spectrophotometric method were proposed for the simultaneous analysis of Drotaverine HCl and Nimesulide in a tablet formulationby absorbance ratio method at 278 nm (isoabsorptive point) and 304 nm(λ max of Drotaverine HCl ) with mean percentage recovery 98.25%±0.66 and 100.5%±0.95, respectively. Beer's law for both methods was obeyed in the concentration range 5-25μg/ml of drotaverine HCl and Nimesulide respectively. The suggested method were successfully applied for the analysis of the two drugs in their pharmaceutical formulation. The obtained results were statistically agreed with those obtained by the reported method.

Keywords

Drotaverine HCl, Nimesulide, Absorbance Ratio Method.

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