Development and Validation of Advance Simultaneous Equation Methods for Lidocaine Hydrochloride and Clotrimazole in Ear Drop

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Lidocaine Hydrochloride and Clotrimazole in Ear Drop by Advance simultaneous equation method. A zero order spectrum was recorded and convert in ratio second order derivative. The Lidocaine Hydrochloride shows max absorbance at 243.20 nm and Clotrimazole show max absorbance at 225 nm. The method was found to be linear (r²>0.999) in the concentration range of 20-100 μg/ml for Lidocaine Hydrochloride and 10-50 μg/ml for Clotrimazole. The limit of determination was 0.2498 μg/ml and 0.0837 μg/ml for Lidocaine Hydrochloride and Clotrimazole, respectively. The limit of quantification was 0.7570 μg/ml and 0.2537 μg/ml for Lidocaine Hydrochloride and Clotrimazole, respectively. The accuracy of this method was evaluated by recovery studies and good recovery result was obtained greater than 99%. The method was successfully applied for simultaneous determination of Lidocaine Hydrochloride and Clotrimazole in Ear Drop.