Asian Journal of Pharmaceutical Research

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Analysis of the Central and Peripheral Mechanisms Underlying the Analgesic Effects of the Extracts of Phyllanthus Amarus and Phyllanthus Fraternus


Affiliations
1 Dept. of Pharmacology and Pharmacognosy, Government College of Pharmacy, Karad, Dist. Satara. 415124
     

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Ethnopharmacological relevance: A great number of preclinical and clinical studies have not only confirmed but have also extended the medicinal uses of species of the genus Phyllanthus mentioned in traditional medicine.

Aim of the study: To evaluate the central and peripheral mechanisms underlying the analgesic effects of the extracts of Phyllanthus amarus and Phyllanthus fraternus in mice using the acetic acid-induced writhing and hot plate tests.

Materials and methods:
a) Writhing was induced by intraperitoneal injection of 0.6% acetic acid (v/v) (80 mg/kg body wt.) and the number of muscular contractions were counted for 30 min following acetic acid injection with animals treated with the extracts of Phyllanthus amarus and Phyllanthus fraternus.
B) The assay was performed according to the classical hot-plate technique of Eddy and Leimbach. The parameter evaluated was the latency time for paw licking and jumping responses after exposure to the hot plate surface.

Results: Results showed that the extracts of Phyllanthus amarus and Phyllanthus fraternus given intraperitoneally can significantly attenuate acetic acid-induced writhing in mice in a dose-dependent manner. In the hot plate latency test, Phyllanthus extracts showed common activity in prolonging duration time and caused marked inhibition of acetic acid induced pain.

Conclusions: These findings of the current study imply the involvement of both peripheral and central antinociceptive mechanisms.


Keywords

Phyllanthus Amarus, Phyllanthus Fraternus, Analgesic Activity, Writhing Test And Hot Plate Test
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  • Calixto, J.B., Santos, A.R.S., Cechinel Filho, V., Yunes, R.A., A review of the plants of the genus Phyllanthus: their chemistry, pharmacology and therapeutic potential. Medicinal Research Review 1998, 18, 225–258.

  • Bagalkotkar G, Sagineedu SR, Saad MS, Stanslas J., Phytochemicals from Phyllanthus niruri Linn. and their pharmacological properties: a review. J Pharm Pharmacol. 2006, 58, 1559-70.

  • Jay Ram Patel, Priyanka Tripathi, Vikas Sharma, Nagendra Singh Chauhan, Vinod Kumar Dixit, Phyllanthus amarus: Ethnomedicinal uses, phytochemistry and pharmacology: A review Journal of Ethnopharmacology, 2011, 138, 286– 313.

  • Santos A. R. S., Cechinel Filho V., Yunes R. A. and Calixto J. B. (1995) Further studies on the antinociceptive action of the hydroalcoholic extracts from plants of the genus Phyllanthus. J. Pharm. Pharmac. 47, 66-71.

  • A.R.S. Santos, V. Cechinel Filho, R.A. Yunes, J.B. Calixto, Analysis of the Mechanisms Underlying the Antinociceptive Effect of the Extracts of Plants From the Genus Phyllanthus, Gen. Pharmac. 1995, 26, 1499-1506,

  • Santos Adair R.S., Rafael O.P. De Campos, Obdulio G. Miguel, Valdir Cechinel Filho, Antonio Carlos Siani, Rosendo A. Yunes, Joao B. Calixto Antinociceptive properties of extracts of new species of plants of the genus Phyllanthus (Euphorbiaceae), Journal of Ethnopharmacology, 2000, 72, 229–238.

  • Candida A.L. Kassuyaa, Aline A. Silvestreb, Vera Lucia G. Rehderb, Joao B. Calixto, Anti-allodynic and anti-oedematogenic properties of the extract and lignans from Phyllanthus amarus in models of persistent inflammatory and neuropathic pain, European Journal of Pharmacology, 2003, 478, 145– 153.

  • Kiemer AK, Hartung T, Huber C and Vollmar AM. Phyllanthus amarus has anti-inflammatory potential by inhibition of iNOS, COX-2, and cytokines via the NF-kappaB pathway. J Hepatol. 2003, 38, 289-97.

  • Chopade, A. R., Somade, P. M. and Sayyed F. J., Membrane stabilizing activity and protein denaturation a possible mechanism of action for the anti-inflammatory activity of Phyllanthus amarus, communicated to JKIMSU, 2012,1, 67-72.

  • R. Koster, M. Anderson, E.J. De Beer, Acetic acid for analgesic screening, Fed. Proc. 1959, 18, 412

  • Ghosh MN. Fundamentals of Experimental Pharmacology. 2 nd rev. ed. Calcutta: Scientific Book Agency; 1984. p. 144-58.

  • N.B. Eddy, D. Leimbach, Synthetic analgesics II. Dithienylbutenyl and dithienylbutylamines, J. Pharmacol. Exp. Ther. 1953, 45, 339.

  • Beatrice Tita, Hanin Abdel-Haq, Annabella Vitalone, Gabriela Mazzanti, Luciano Saso, Analgesic properties of Epilobium angustifolium, evaluated by the hot plate test and the writhing test, Farmaco 2001, 56, 341–343.

  • Deraedt R, Jouquey S, Delevallee F, Flahaut M (1980) Release of prostaglandins E and F in an algogenic reaction and its inhibition. Eur J Pharmacol 61: 17–24

  • Borsato MLC, Grael CFF, Souza GEP, Lopes NP Analgesic activity of the lignans from Lychnophora ericoides. Phytochemistry 2000, 55: 809–813.

  • Duarte JDG, Nakamura M, Ferreira SH (1988) Participation of the sympathetic system in acetic acid induced writhing in mice. Brazilian J Med Res 21: 341–343


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  • Analysis of the Central and Peripheral Mechanisms Underlying the Analgesic Effects of the Extracts of Phyllanthus Amarus and Phyllanthus Fraternus

Abstract Views: 895  |  PDF Views: 2

Authors

Atul R. Chopade
Dept. of Pharmacology and Pharmacognosy, Government College of Pharmacy, Karad, Dist. Satara. 415124
F. J. Sayyad
Dept. of Pharmacology and Pharmacognosy, Government College of Pharmacy, Karad, Dist. Satara. 415124

Abstract


Ethnopharmacological relevance: A great number of preclinical and clinical studies have not only confirmed but have also extended the medicinal uses of species of the genus Phyllanthus mentioned in traditional medicine.

Aim of the study: To evaluate the central and peripheral mechanisms underlying the analgesic effects of the extracts of Phyllanthus amarus and Phyllanthus fraternus in mice using the acetic acid-induced writhing and hot plate tests.

Materials and methods:
a) Writhing was induced by intraperitoneal injection of 0.6% acetic acid (v/v) (80 mg/kg body wt.) and the number of muscular contractions were counted for 30 min following acetic acid injection with animals treated with the extracts of Phyllanthus amarus and Phyllanthus fraternus.
B) The assay was performed according to the classical hot-plate technique of Eddy and Leimbach. The parameter evaluated was the latency time for paw licking and jumping responses after exposure to the hot plate surface.

Results: Results showed that the extracts of Phyllanthus amarus and Phyllanthus fraternus given intraperitoneally can significantly attenuate acetic acid-induced writhing in mice in a dose-dependent manner. In the hot plate latency test, Phyllanthus extracts showed common activity in prolonging duration time and caused marked inhibition of acetic acid induced pain.

Conclusions: These findings of the current study imply the involvement of both peripheral and central antinociceptive mechanisms.


Keywords


Phyllanthus Amarus, Phyllanthus Fraternus, Analgesic Activity, Writhing Test And Hot Plate Test

References