A Novel System for Drug Delivery Using Nanoparticle

Several biological drugs such as proteins and nucleic acids require novel delivery technologies that will minimize side effects and lead to better patient compliance. Hence, various research using nanoparticle as a carrier has been done for the delivery of gene drug. In the present work, delivery of proteases and plasmid DNA using nanoparticle as a carrier on E.colis was studied. Proteases, a proteolytic enzyme which is capable of cleaving the peptides are made inactive by inhibitors present inside the cell. But proteases can be made resistive by coating with nanoparticles. The action of protease has been analysed on a specific enzyme i.e. alkaline phosphatase. Along with the protease delivery, plasmid delivery is also done to develop a carrier capable of delivering drug and gene complex. Similarly, carrier system for drug and plasmid was studied and developed for cancer cells which were capable of delivering drugs as well as short polynucleotide complex. It was found that the cancer cells lines SW480 and A549 showed cell death due to the action of drugs. This novel carrier showed effective delivery of drug and high efficacy in action. From this study it was confirmed that a protein or drug can be conjugated with nanoparticles along with gene carrier to import both functions of drugs and gene on a targeted cell using single carrier. Such system can be developed specific genetic disorders such as Osteogenesis imperfecta, cancer.