Simple and Rapid Spectrofluorometric Fingerprint Development of Quinine in Unani Formulation

A simple, precise and accurate spectrofluorometric method has been established for quantitative determination of quinine. Conditions were also optimized for best possible extraction of quinine via varying concentrations of different solvents for maximum recovery of quinine at excitation wavelength 372 nm and emission wavelength 456 nm. Methanol found to be best for highest possible recovery of target analyte quinine. The linear range of 1.0- 7.0 μg/mL was obtained for quinine. The detection limit was 0.1768 and quantitation limit was found to be 0.5411for quinine. The recovery of the quinine was found to be 97.35-99.85 %. The proposed method was used for the determination of quinine alkaloids in pharmaceuticals and herbal formulations with satisfactory result.