Simultaneous Estimation of Diethylcarbamazine Citrate and Cetirizine Hydrochloride in Formulation by RP-HPLC

A Simple and reproducible method for simultaneous estimation of Diethylcarbamazine citrate (DECC) and Cetirizine hydrochloride (CTH) by High performance liquid chromatography (HPLC) in formulation was developed. The analysis was performed with a mobile phase containing Acetonitrile and mixed phosphate buffer (KH₂PO₄ and KH₂PO₄, adjusted to pH - 3.0 with 10% orthophosphoric acid) in the ratio of 60:40(%v/v) at a flow rate of 1ml/min. A phenomenex C₁₈ column (250 × 4.6mm,5μm)was used. The wavelength of DECC is 216nm and Cetirizine is 230nm and from the overlapping spectra a wavelength of 220nm is selected and simultaneous estimation is carried out .The analysis was performed in the linearity range of 24-144 μg/ml for Diethylcarbamazine citrate and 0.8 - 4.8 μg/ml for Cetirizine hydrochloride and the minimum detectable level was 0.62 μg/ml and 0.105 μg/ml.The correlation coefficient was 0.998 and 0.997.Thus the developed and validated chromatographic method for Diethylcarbamazine citrate and Cetirizine hydrochloride is said to be rapid, simple, accurate and cost effective that can be applied for routine analysis in research institution, quality control department in industries and in clinical pharmacokinetic studies.