Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Validation of Oxolamine Citrate by Derivative UV Spectrophotometric Method in Pharmaceutical Dosage Form


Affiliations
1 Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016, India
     

   Subscribe/Renew Journal


A simple and precise third order derivative method have been developed and validated for the estimation of oxolamine citrate in bulk and its tablet formulation. Oxolamine citrate was estimated at 254.6 nm for the third order derivative UV-spectrophotometric method. Beer's law was obeyed in the concentration range of 1 to 14 μg / ml with coefficient of correlation value 0.9995 for third order derivative method. The method was tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation, which was as 2.0487 % for the above method. The proposed method was successfully applied for the determination of oxolamine citrate in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.

Keywords

Oxolamine Citrate, Third Order Derivative Spectroscopy.
Subscription Login to verify subscription
User
Notifications
Font Size


Abstract Views: 229

PDF Views: 2




  • Validation of Oxolamine Citrate by Derivative UV Spectrophotometric Method in Pharmaceutical Dosage Form

Abstract Views: 229  |  PDF Views: 2

Authors

V. Rele Rajan
Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016, India

Abstract


A simple and precise third order derivative method have been developed and validated for the estimation of oxolamine citrate in bulk and its tablet formulation. Oxolamine citrate was estimated at 254.6 nm for the third order derivative UV-spectrophotometric method. Beer's law was obeyed in the concentration range of 1 to 14 μg / ml with coefficient of correlation value 0.9995 for third order derivative method. The method was tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation, which was as 2.0487 % for the above method. The proposed method was successfully applied for the determination of oxolamine citrate in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.

Keywords


Oxolamine Citrate, Third Order Derivative Spectroscopy.