UV-Spectrophotometry Method for the Estimation of Linagliptin in Bulk and Pharmaceutical Formulations

The main objective was to develop and validate the UV-spectrophotometric method for the estimation of linagliptin in bulk and pharmaceutical formulations as per ICH guidelines, acetonitrile was used as solvent. The λ_max of linagliptin was found to be 296 nm it was proved linearity in the concentration range 1-10 μg/ml with a correlation coefficient value of 0.999. The accuracy studies of proposed method was performed at three different levels, i.e., 50%, 100% and 150% and recovery was found to be in the range of 100.4%. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.23 and 0.73 μg/ml, respectively. The % RSD less than 2 which indicates the accuracy and precise of the method. The above method was a rapid tool for routine analysis of linagliptin in the bulk and in the pharmaceutical dosage form.