HPTLC Estimation of Cefixime and Cloxacillin in Tablet Dosage Form

A simple, rapid, reliable and accurate HPTLC method has been developed for the quantitative determination of Cefixime and Cloxacillin in bulk and tablets. The drugs were extracted from (Zifi×200). Various aliquots of this sample solution were spotted automatically by means of Camag (Muttenz;Switzerland) Linomat V sample applicator on Merck HPTLC plates (0.2 mm thickness) precoated with silica gel 60 F₂₅₄ on aluminium sheet as stationary phase prewashed with methanol using n-Butanol: Methanol: Water: Formic acid (8:6:4:0.3 v/v/v) as mobile phase. The spots were scanned at λ=293 and 343 nm for Cefixime and Cloxacillin respectively using Camag TLC scanner 3. The R_f values of Cefixime and Cloxacillin were found to be 0.28 and 0.45 respectively. Calibration curves were linear in range of 150-600 ng per spot. The limit of detection (LOD) and quantitation (LOQ) for Cefixime and Cloxacillin were found to be 50, 20 and 150, 60 ng per spot respectively. The suitability of this method for quantitative determination of compounds was proved by validation in accordance with requirements of pharmaceutical regulatory standards. The proposed method is valid, simple, sensitive and accurate. Therefore this method can be applied for routine analysis of these drugs in bulk and tablet formulation.