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Derivatized HPTLC Method for Simultaneous Estimation of Glucosamine, Vitamin C and Vitamin E in Tablets


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1 Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
     

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An accurate, precise and derivatized HPTLC method has been developed for the simultaneous estimation of Glucosamine, Vitamin C and Vitamin E in tablet formulation. In this method standard and sample solutions of Glucosamine, Vitamin C and Vitamin E were applied on pre-coated silica gel 60F254 TLC plate, and developed using ethanol:Acetic acid (9:1 v/v), as mobile phase and derivatized using Iodine vapor. The drugs on the plate were scanned at 500 nm. The dynamic linearity range was 20-100 μg/spot for Glucosamine, 2-10 μg/spot for Vitamin C and 0.2-1 μg/spot for Vitamin E. The method was validated for precision, accuracy and reproducibility.

Keywords

Simultaneous Estimation, HPTLC, Glucosamine, Vitamin C and Vitamin E.
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  • Derivatized HPTLC Method for Simultaneous Estimation of Glucosamine, Vitamin C and Vitamin E in Tablets

Abstract Views: 240  |  PDF Views: 0

Authors

W. D. Sam Solomon
Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
Rahul A. Kumar
Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
P. R. Vijai Anand
Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India
R. Venkatnarayanan
Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, India

Abstract


An accurate, precise and derivatized HPTLC method has been developed for the simultaneous estimation of Glucosamine, Vitamin C and Vitamin E in tablet formulation. In this method standard and sample solutions of Glucosamine, Vitamin C and Vitamin E were applied on pre-coated silica gel 60F254 TLC plate, and developed using ethanol:Acetic acid (9:1 v/v), as mobile phase and derivatized using Iodine vapor. The drugs on the plate were scanned at 500 nm. The dynamic linearity range was 20-100 μg/spot for Glucosamine, 2-10 μg/spot for Vitamin C and 0.2-1 μg/spot for Vitamin E. The method was validated for precision, accuracy and reproducibility.

Keywords


Simultaneous Estimation, HPTLC, Glucosamine, Vitamin C and Vitamin E.