Estimation of Levetiracetam in Tablet by UPLC

A rapid, precise, accurate and specific ultra performance liquid chromatography method was developed for the estimation of levetiracetam in tablet dosage form. Bridged Ethyl Hybrid (BEH), C 18, 100*2.1 mm, 1.7 μ/CLC 253 column, with mobile phase consisting of 0.3% w/v orthophosphoric acid and methanol in the ratio of 80:20 v/v was used. The flow rate was 1 ml/min and the effluents were monitored at 210 nm. The retention time was 1.55 min and the detector response was linear in the concentration range of 50-150 μg/ml with the linear regression equation being Y=12327x+7411. The limit of detection and limit of quantification was 0.00675 and 0.0225 μg/ml respectively. The percentage assay of Levetiracetam was 99.57±0.831%. The method was validated in terms of accuracy and precision. The results of the study showed that the proposed ultra performance liquid chromatography (UPLC) method is useful for the routine determination of levetiracetam in its pharmaceutical tablet dosage form.