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RP-HPLC Determination of Atenolol in its Pharmaceutical Dosage Forms


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1 People’s Institute of Pharmacy and Research Centre, Bhanpur, Bhopal-462037 (M.P.), India
     

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A new and simple reverse phase HPLC method was developed for the estimation of atenolol in its pharmaceutical dosage forms. The mobile phase used acetonitrile, methanol, KH2PO4 is in the ratio of 250:250:500. Buffer solution was prepared by dissolving 0.05 M KH2PO4+0.1% H3PO4 adjunct with triethylamine. The separation was achieved on hypersil C-18 column, phenomenex Gemini(250×4.6 mm) and 5 μ particle size with rheodyne injector. The flow rate was 1 ml/min and uv detection at 238 nm. The retention time for atenolol was 4 min respectively. The linearity coefficient of atenolol was found to be 0.99% and the percentage recoveries for atenolol is 99.89%. The proposed method was accurate, precise and linear within the desired range. This method can successfully employed for the quantitative analysis of atenolol.

Keywords

RP HPLC, Validation, Atenolol.
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  • RP-HPLC Determination of Atenolol in its Pharmaceutical Dosage Forms

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Authors

Amrish Sharma
People’s Institute of Pharmacy and Research Centre, Bhanpur, Bhopal-462037 (M.P.), India
Mukul Tailang
People’s Institute of Pharmacy and Research Centre, Bhanpur, Bhopal-462037 (M.P.), India
Sonali Khare
People’s Institute of Pharmacy and Research Centre, Bhanpur, Bhopal-462037 (M.P.), India
Tripti Shukla
People’s Institute of Pharmacy and Research Centre, Bhanpur, Bhopal-462037 (M.P.), India

Abstract


A new and simple reverse phase HPLC method was developed for the estimation of atenolol in its pharmaceutical dosage forms. The mobile phase used acetonitrile, methanol, KH2PO4 is in the ratio of 250:250:500. Buffer solution was prepared by dissolving 0.05 M KH2PO4+0.1% H3PO4 adjunct with triethylamine. The separation was achieved on hypersil C-18 column, phenomenex Gemini(250×4.6 mm) and 5 μ particle size with rheodyne injector. The flow rate was 1 ml/min and uv detection at 238 nm. The retention time for atenolol was 4 min respectively. The linearity coefficient of atenolol was found to be 0.99% and the percentage recoveries for atenolol is 99.89%. The proposed method was accurate, precise and linear within the desired range. This method can successfully employed for the quantitative analysis of atenolol.

Keywords


RP HPLC, Validation, Atenolol.