Development and Validation of Method for Simultaneous Estimation of Atenolol and Lercanidipine from Tablet Dosage Form by Second Order Derivative Spectroscopy

Atenolol and Lercanidipine are used in combination for treatment of hypertension. The present work deals with simple spectrophotometric method development for simultaneous estimation of Atenolol (ATN) and Lercanidipine (LER) in tablet formulation. The method employed second order derivative spectroscopy. For determination of sampling wavelength 10 μg/ml of each of ATN and LER were scanned in 200-350 nm range and sampling. The values of D amplitudes were measured 275 nm (zero crossing of LER) and 322 nm (zero crossing of ATN) for the determination of ATN and LER, respectively. For this method linearity was observed in 10-60 μg/ml for ATN and 10-60 μg/ml for LER. The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of method. The method is validated as per ICH guidelines.