Asian Journal of Research in Chemistry

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Evaluation of Antioxidant Capacity by Cyclic Voltammetry of Phoenix dactylifera L. (date palm)


Affiliations
1 Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria., India
2 Department of Exact Sciences, Higher Normal School of Ouargla, 30000 Ouargla, Algeria., India
3 Department of Exact Sciences, Higher Normal School of Ouargla, 30000 Ouargla, Algeria, India
4 Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria, India
5 Department of Agronomy, Faculty of Nature and Life Sciences and Earth Sciences, University of Ghardaia, 47000 Ghardaia, Algeria., India
     

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In this study we used spectrophotometrical techniques to estimate antioxidant efficacy by using 2,2-diphenyl-1- picrylhydrazyl free radical scavenging capacity and reducing power capacity. We have used also electrochemical techniques performed by cyclic voltammetry. The present study examined the antioxidant activities of the methanolic extract (ME) of date palm (Phoenix dactylifera. L) fruits (DPF) of four native cultivars from Algeria, namely, Ghars (Gh), Chtaya (Cht), Degla Beïda (DB) and Tinissine (Tns). Quantitative estimation of total phenol and flavonoid content by a colorimetric assay showed that methanol extracts are rich in these components. The content of total phenolics of different cultivars extracts was in the range of 26.083 - 35.850 GAE/100g DW. while it was the content of total flavonoids of the different cultivars extracts was in the range of 5.276 - 6.055 QE/100g DW. The value of IC50 for DPPH radical test ranged from 0.193 to 0.400mg/ml. All extracts showed very good activity of ferric reducing power, the higher power was in methanolic extract of Tns. The less IC50 value in cyclic voltammetry method (CV), which meet the highest effective antioxidant was 0.121 mg/ml in methanolic extract of Tns.

Keywords

Phoenix dactylifera L, Phenolic content, Flavonoids contents, antioxidant activity, cyclic voltammetry.
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  • Evaluation of Antioxidant Capacity by Cyclic Voltammetry of Phoenix dactylifera L. (date palm)

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Authors

Assia Belfar
Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria., India
Assia Belfar
Department of Exact Sciences, Higher Normal School of Ouargla, 30000 Ouargla, Algeria., India
Cheyma Bensaci
Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria., India
Cheyma Bensaci
Department of Exact Sciences, Higher Normal School of Ouargla, 30000 Ouargla, Algeria, India
Mahdi Belguidoum
Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria, India
Mahdi Belguidoum
Department of Agronomy, Faculty of Nature and Life Sciences and Earth Sciences, University of Ghardaia, 47000 Ghardaia, Algeria., India
Mokhtar Saidi
Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria., India
Zineb Ghiaba
Laboratory of Valorization and Promotion of Saharan Resources, Kasdi Merbah University, 30000 Ouargla, Algeria., India

Abstract


In this study we used spectrophotometrical techniques to estimate antioxidant efficacy by using 2,2-diphenyl-1- picrylhydrazyl free radical scavenging capacity and reducing power capacity. We have used also electrochemical techniques performed by cyclic voltammetry. The present study examined the antioxidant activities of the methanolic extract (ME) of date palm (Phoenix dactylifera. L) fruits (DPF) of four native cultivars from Algeria, namely, Ghars (Gh), Chtaya (Cht), Degla Beïda (DB) and Tinissine (Tns). Quantitative estimation of total phenol and flavonoid content by a colorimetric assay showed that methanol extracts are rich in these components. The content of total phenolics of different cultivars extracts was in the range of 26.083 - 35.850 GAE/100g DW. while it was the content of total flavonoids of the different cultivars extracts was in the range of 5.276 - 6.055 QE/100g DW. The value of IC50 for DPPH radical test ranged from 0.193 to 0.400mg/ml. All extracts showed very good activity of ferric reducing power, the higher power was in methanolic extract of Tns. The less IC50 value in cyclic voltammetry method (CV), which meet the highest effective antioxidant was 0.121 mg/ml in methanolic extract of Tns.

Keywords


Phoenix dactylifera L, Phenolic content, Flavonoids contents, antioxidant activity, cyclic voltammetry.

References