Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Application of RP-HPLC Technique for Development of Analytical Method for Validation of Albuterol Sulphate from Bulk Drug and Dosage Form


Affiliations
1 Central Research Laboratory, D. G. Ruparel College, Matunga, Mumbai 400016 ., Qatar
2 Central Research Laboratory, D. G. Ruparel College, Matunga, Mumbai 400016., India
     

   Subscribe/Renew Journal


A novel reverse phase liquid chromatographic method was developed and validated for estimation of Albuterol sulphate in its dosage form i.e. tablets. The reverse phase HPLC analysis was carried out on isocratic system. The column was Hypersil BDS C18 (150mm x 4.6mm, 5µm) with ambient temperature. The mobile phase consisted of buffer: methanol in proportion 75:25 % (v/v). The flow rate was maintained at 1.0ml/min. The detection was carried out at wavelength 225nm. The method was validated as per ICH guidelines for system suitability, linearity, accuracy and precision. The linear ranges were 50-150µg/ml for albuterol sulphate, . The accuracy and precision were found to be well within the acceptable limit. The method was successfully applied for determination albuterol sulphate in dosage form with good recoveries.

Keywords

Albuterol sulphate, Tri ethyl amine, Phosphoric acid, Methanol, HPLC
Subscription Login to verify subscription
User
Notifications
Font Size


  • Mohauman Mohammad AL-Rufaie, Aymen Abdul Rasool Jawad, Hawraa Mohammed Sadiq. Colorimetric estimation for salbutamol sulphate in pure form and in different types of pharmaceutical.International Journal of ChemTech Research, 9(11); 2016: 432-441.

  • L. Kalyani a, Chava V.N. Rao. Simultaneous spectrophotometric estimation of Salbutamol, Theophylline and Ambroxol three component tablet formulation using simultaneous equation methods. International Journal of Modern Science. 4 ;2018: 171-179.

  • Kimberley Marita Dias, Padmavathi P. Prabhu. Development of new analytical method and its validation for the determination of salbutamol sulphate in bulk and marketed formulations. International Journal of Pharmacy and Pharmaceutical Sciences. 7(6); 2015: 397-400.

  • Mohauman Mohammad AL-Rufaie, Aymen Abdul Rasool Jawad, Hawraa Mohammed Sadiq. Colorimetric Estimation for Salbutamolsulphate in Pure Form and in Different Types of Pharmaceutical. International Journal of ChemTech Research. 2015; 1(1): 432-441.

  • Parth R. Joshi, Shraddha J. Parmar, and Bhavna A. Patel.Spectrophotometric Simultaneous Determination of Salbutamol Sulfate and Ketotifen Fumarate in Combined Tablet Dosage Form by FirstOrder Derivative Spectroscopy Method. International Journal of Spectroscopy. 2013, Article ID 589218, 6 pages, http://dx.doi.org/10.1155/2013/589218.

  • Kanakapura Basavaiah, Bankavadi Chikkaswamy Somashekar, Veeraiah Ramakrishna. Rapid titrimetric and spectrophotometric methods for salbutamol sulphate in pharmaceuticals using Nbromosuccinimide, Acta Pharm. 57;2007: 87–98.

  • Rele R.V. Simultaneous Spectrophotometric Estimation of Ambroxal hydrochloride and Salbutamol sulphate by First Order derivative Method in Combined Dosage Form. Asian J. Research Chem. 8(4): 2015; 273-277.

  • Rajan V. Rele. UV- Spectrophotometric Third Order derivative Method for Simultaneous Estimation of Ambroxal hydrochloride and Salbutamol sulphate by in Combined pharmaceutical Dosage Form.

  • Der Pharmacia Lettre. 8 (3); 2016:261-266.

  • Rajan V. Rele. Simultaneous Spectrophotometric Estimation of Ambroxal Hydrochloride and Salbutamol Sulphate by Second Order Derivative Method in Combined Dosage Form. Research J. Pharm. and Tech. 8(4); 2015: 432-436.

  • Rajan V. Rele. Simultaneous determination of bromhexine hydrochloride and salbutamol sulphate in pharmaceutical dosage by reverse phase high performance liquid chromatography. Der Pharmacia Lettre. 7 (3); 2015:157-161.

  • Rajan V. Rele. Simultaneous UV-spectrophotometric estimation of bromhexine hydrochloride and salbutamol sulphate by area under curve method in combined dosage form. Der Pharmacia Sinica. 2015, 6(5):8-14.

  • Rajan V. Rele. Simultaneous UV-spectrophotometric estimation of bromhexine hydrochloride and salbutamol sulphate by first order derivative method in combined dosage form. Journal of Chemical and Pharmaceutical Research. 7(5); 2015:265-270.

  • Rajan V. Rele. Simultaneous UV-Spectrophotometric Estimation of Bromhexine Hydrochloride and Salbutamol Sulphate by Second Order Derivative Method in Combined Dosage Form. Research J. Pharm. and Tech. 8(6); 2015:702-706.

  • Rajan V. Rele. Simultaneous UV-spectrophotometric estimation of bromhexine hydrochloride and salbutamol sulphate by third order derivative Method in combined pharmaceutical dosage form. Journal of Chemical and Pharmaceutical Research. 8(3); 2016:289-294.

  • B.C.Somashekar, V. Ramakrishna. Titrimetric And Spectrophotometric Determination Of Salbutamol Sulphate In Pharmaceuticals Using Chloramine-T And Two Dyes. Analytical Chemistry, An Indian Journal. 2(5,6); 2006:179-186.

  • Sagar Suman Panda, Bera Venkata Varaha Ravi Kumar, Ganeswar Mohanta. Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form. Brazilian Journal of Pharmaceutical Sciences. 49(3); 2013: 475-490.

  • Sawant Ramesh L., Bharat Anjali V., Tanpure Kallyani D., Jadhav Kallyani A. A new RP-HPLC method development for simultaneous estimation of salbutamol sulphate, theophylline and furosemide.

  • International Journal of Pharma Sciences and Research. 6(4); 2015: 683-688.

  • Nidhi Dubey, Sandip Sahu, G.N. Singh. Development of HPLC method for simultaneous estimation of ambroxal guaiphensin and salbutamol in single dosage form. International Journal of Chemistry.

  • (B);2012: 1633-1636.

  • Mohammad Abdul Motalib Momin, Md. Faisal Hossain, Anjuman Ara Begum, Jhani Roy, Sharif Md. Anisuzzaman. Development and Validation of Stability Indicating Assay Method of Salbutamol Sulphate Metered Dose Inhaler by HPLC. International Journal of Pharmaceutical and Phytopharmacological Research. 2 (6);2013: 439448.

  • G. Sowjanya, D. Gowri Sankar J. V. L. N. Seshagiri Rao. Development and Validation of a new RP-HPLC method for the simultaneous determination of albuterol sulphate and Ipratropium bromide in nasal

  • inhalations. International Research Journal of Pharmacy. 9 (8); 2018:63-70.

  • Rajan V. Rele. Simultaneous determination of Ambroxal hydrochloride and Salbutamol Sulphate in pharmaceutical dosage by reverse phase high performance liquid chromatography. Journal of Chemical and

  • Pharmaceutical Research. 7(2); 2015:802-806.

  • Rele Rajan V. Simultaneous determination of ambroxal hydrochloride,guaiphenesin and salbutamol sulphate in pharmaceutical dosage by reverse phase high performance liquid chromatography. Der Pharmacia Lettre. 7 (5); 2015:44-49.

  • Niyazi Yilmaz, Sibel A. Ozkan, Bengi Uslu Zuhre S_enturk, and _Inci biryol. Voltammetric Determination of Salbutamol Based on Electrochemical Oxidation at Platinum and Glassy Carbon Electrodes.

  • Tr. J. of Chemistry. 22; (1998):175 -182.

  • Pangal Anees. Simple Titrimetric Method for the Estimation of Salbutamol Sulphate (SBS) in Pharmaceutical Formulations. Research Journal of Pharmaceutical Sciences. 2(1), 2013,11-14.


Abstract Views: 133

PDF Views: 0




  • Application of RP-HPLC Technique for Development of Analytical Method for Validation of Albuterol Sulphate from Bulk Drug and Dosage Form

Abstract Views: 133  |  PDF Views: 0

Authors

Rajan V. Rele
Central Research Laboratory, D. G. Ruparel College, Matunga, Mumbai 400016 ., Qatar
Pratamesh P. Tiwatane
Central Research Laboratory, D. G. Ruparel College, Matunga, Mumbai 400016., India
Sandip P. Patil
Central Research Laboratory, D. G. Ruparel College, Matunga, Mumbai 400016., India

Abstract


A novel reverse phase liquid chromatographic method was developed and validated for estimation of Albuterol sulphate in its dosage form i.e. tablets. The reverse phase HPLC analysis was carried out on isocratic system. The column was Hypersil BDS C18 (150mm x 4.6mm, 5µm) with ambient temperature. The mobile phase consisted of buffer: methanol in proportion 75:25 % (v/v). The flow rate was maintained at 1.0ml/min. The detection was carried out at wavelength 225nm. The method was validated as per ICH guidelines for system suitability, linearity, accuracy and precision. The linear ranges were 50-150µg/ml for albuterol sulphate, . The accuracy and precision were found to be well within the acceptable limit. The method was successfully applied for determination albuterol sulphate in dosage form with good recoveries.

Keywords


Albuterol sulphate, Tri ethyl amine, Phosphoric acid, Methanol, HPLC

References