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Simultaneous Estimation of Artemether and Lumefantrine in Pharmaceutical dosage forms using Derivative Spectrophotometry
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In the present investigation a simple, rapid and accurate method for the simultaneous estimation of artemether (ART) and lumefantrine (LUM) was developed using derivative spectroscopic technique. To avoid the overlapping of ART and LUM in zero order spectra, first order spectrum was developed. ART showed good linearity with regression coefficient (r<SUP>2</SUP>) of 0.9993 over the range of 30-80μg/ml at 260 nm wavelength and LUM showed linearity over the range of 4-12µg/ml at 286.2 nm wave length with correlation coefficient (r<SUP>2</SUP> ) of 0.9999. The analytical method was validated for simultaneous estimation of ART and LUM both as drug solutions, in tablets and in NLCs and validation parameters are reported. Intercept value of the linear lines determined for ART and LUM were nearly zero. ART and LUM showed accuracy of 99.215 and 99.564 in terms of % recovery. The relative standard deviation (RSD at n=6) intra-day: Inter-day precision for ART and LUM were 0.944:1.124 and 0.890:1.009 respectively indicating the method is precise. LOD (µg/ml) and LOQ (μg/ml) values for ART were 0.3606 and 1.0928 and for LUM were 0.1145 and 0.3470 respectively. The developed method was successfully applied in the estimation of ART and LUM in commercial tablet (Arte plus CD) containing both the drugs.
Keywords
Artemether, Lumefantrine, Derivative Spectroscopy, Simultaneous Estimation, Validation
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- Muhia DK, Mberu EK, Wat kins WM. Differential extraction of artemether and its metabolite dihydroartemisinin from plasma and determination by high-performance liquid chromatography .J. Chromatogr. B 660 (1994) 196–199.
- Karbwang J, Na-Bangchang K, Molunto P, Banmairuroi V, Congpuong K. Pharmacokinetics of intramuscular artemether in patients with severe falciparum malaria with or without acute renal failure. J. Chromatogr. B 690 (1997) 259–265.
- Meshnick SR, Taylor TE, Kamchonwongpaisan S, Artimisinin and the antimalarial endoperoxides: From herbal remedy to targeted chemotherapy, Microbiological review, 1996, 60(2), 301-15.
- Chimanuka, B., Gabriels, M., Detaevernier, M.R., Plaizier- Vercammen, J.A., 2002 Preparation of beta-artemether liposomes, their HPLC-UV evaluation and relevance for clearing recrudescent parasitaemia in Plasmodium chabaudi malariainfected mice. J. Pharm. Biomed. Anal. 28, 13–22
- Aditya NP, Patankar S, Madhusudhan B, Murthy RSR, Souto EB. Artemether loaded lipid nanoparticles produced by modified-thin film hydration: Pharmacokinetics, toxicological and in vivo antimalarial activity. European Journal of Pharmaceutical Sciences.2010; 40:448-455.
- Arun R. and Anton S A. Development of analytical method for Lumefantrine by UV-Spectrophtometry. Int. J. Res. Pharm. Sci. 2010;1(3):321-4
- Shrivastava A, Nagori BP, Saini P, Issarani R, Gaur S. New Simple and Economical Spectrophotometric Method for Estimation of Artemether in Pharmaceutical Dosage Forms. Asian J. Research Chem. (2008)1, 1-19.
- Cesar C, Noqueira FH, Antonio P G. Simultaneous determination of Artemether and Lumefantrine in fixed dose combination tablets by HPLC with UV detection. . J. Pharm. Biomed. Anal. 48(1)10 2008, Pages 223–226
- Thomas CG, Ward SA, Edwards G. Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection. J. Chromatogr. B 583 (1992) 131–136.
- Khalil IF, Abildrup U, Alifrangis LH, Magia D, Alifrangis M, Hoegberg L, Vestergaarde LS, Persson OP, Nyagonde N, Lemnge MM, Theander TG, Bygbierg IC. developed the measurement of Lumefantrine and its metabolite in plasma by HPLC with UV detection. J Pharm Biomed Anal, 2011 Jan 5;54(1) : 168-72.
- Gabrils M, Plaizier-Vercammen JA,. Densitometric Thin-Layer Chromatographic Determination of Artemisinin and its Lipophilic Derivatives, Artemether and Arteether. J Chromatogr Sci. (2003), 41(7):359-66.
- Gabrils M, Plaizier-Vercammen JA,. Development of a Reversed-Phase Thin-Layer Chromatographic Method for Artemisinin and Its Derivatives, .J. Chromatogr. Sci. 42 (2004) 341–347.
- Verbeken M, Sultan S, Bram B, Vangheluwe E , Sylvia VD, Christian Burvenich, Luc-Duchateau, Frans H J and Bart D S. Stability-indicating HPLC-DAD/UV- EST/MS impurity profiling of the anti-malarial drug Lumefantrine. Malarial J.2011;10: 51.
- Hulst AD, Augustijns P, Arens S, Van Parijs L, Colson S, Verbeke N, Kinget R. Determination of Artesunate by Capillary Electrophoresis with Low UV Detection and possible application to analogues. J. Chromatogr. Sci. 41 (1996)34.
- Varma J K, Syed H. A. Spectrophotometric method for determination of Lumefantrine in pharmaceutical formulation. J. Pharm. Res. 2009;2(9):1550-1.
- Beckett AH, Stenlake JB, Practical Pharmaceutical Chemistry. 4th ed. Delhi: CBS Publisher and Distributors, 1997.
- Patel PM, Suhagia BN, Shah SA, Marolia BP, Bodiwala KB, Prajapati PB, Simultaneous estimation of etoricoxib and paracetamol in the synthetic mixture and Pharmaceutical dosage forms by derivative spectophotometry, Journal of Pharmacy Research, 2010, 3(8), 1967-70.
- Donald W, A Practical handbook of preparative HPLC, Elsevier publisher, New York, 2006, p. 37-45.
- Thomas CG, Ward SA, Edwards G, J. Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection, Chromatogr. 1992, 583, 131-6. Green MD, Mount DL, Wirtz RA., Authentication of artemether, artesunate and dihydroartemisinin antimalarial tablets using a simple colorimetric method., Trop. Med. Int. Health, 2001 Dec;6(12):980-82.
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