Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Spectrophotometric Estimation of Ambroxol Hydrochloride, Guaifenesin and Levosalbutamol Sulphatein Syrup


Affiliations
1 Department of Quality Assurance, Shree S. K. Patel College of Pharmaceutical Education and Research, Ganpat University, Ganpat Vidyanagar – 384012, Dist. – Mehsana (Gujarat), India
     

   Subscribe/Renew Journal


This study proposes a method for estimation of Ambroxol HCl, Guaifenesin and Levosalbutamol sulphate in syrup form. The study was done by combining three spectrophotometric methods viz use of first order derivative and colorimetry. Absorption of Guaifenesin and Levosalbutamol sulphate were found to be zero at 323 nm, thus enabling the measurement of Ambroxol HCl, using specific absorbance in first order spectrum. Same way absorption of Ambroxol HCl and Levosalbutamol sulphate were found to be zero at 276 nm, thus enabling the measurement of Guaifenesin using specific absorbance in first order spectrum. For colorimetric measurement of Levosalbutamol Sulphate, a colored substance was obtained by coupling the oxidized product of Levosalbutamol sulphate with 4- aminoantipyrine and Potassium ferricyanide and its absorption was measured at 503 nm. The proposed method was statistically validated in accordance with ICH guidelines and results were found to be satisfactory for accuracy, precision and specificity.

Keywords

Ambroxol HCL, Guaifenesin And Levosalbutamol Sulphate, First Order Derivative, Colorimetric Method
Subscription Login to verify subscription
User
Notifications
Font Size


  • Rang H., Dale M., Ritter J. and Moore P., Pharmacology, 5 th ed., page no.357-367, 2003.

  • Sweetman S., The Martindale Complete Drug Reference 35th Edition, Pharmaceutical Press, page no .1398, 1168, 527, 2002.

  • Maryadele. J., Merck Index 14th Edition, Merck & Co. Inc, White House Station NJ, page no. 386, 4561, 5578, 2006.

  • Indian Pharmacopoeia. Vol. II. 6thedition, Govt. of India, New Delhi: The Controller of Publication; 82-83, 2010.

  • Senthil M., Raja S., “RP-HPLC method development and validation for the simultaneous estimation of Azithromycin and Ambroxol Hydrochloride in tablet,” International Journal of Pharmtech Research, 2, (1), 36-39, 2008.

  • Krupa M., Kothekara B., Balasundaramjayakar N., “Quantitative determination of Levofloxacin and Ambroxol Hydrochloride in pharmaceutical dosage form by Reversed Phase High Performance Liquid Chromatography,” Eurasian Journal of Analytical Chemistry, 2, (1), 156-162, 2007.

  • Gopala K., “Development of RP-HPLC method for the simultaneous estimation of Ambroxol Hydrochloride, Cetirizine Hydrochloride and Antimicrobial Preservatives in combined dosage form, Journal of Pharmaceutical Science, 4 (3), 1003- 1015, 2003.

  • B. Prathap, S. S. Rajendran, “Simultaneous determination of Gatifloxacin and Ambroxol Hydrochloride from tablet dosage form using RP-HPLC,” Journal of Pharmaceutical Science, 1, (3), 325-327, 2010.

  • Hanimi R., “Stability Indicating RP-HPLC method for the determination of Terbutaline Sulphate, Guaifenesin, Ambroxol Hydrochloride and Preservatives content in liquid formulations,” Journal Of Pharmacy Research,4, (11), 4117-4122, 2011.

  • Lakshmanaprabu S., “Simultaneous determination of Gatifloxacin and Ambroxol Hydrochloride in a tablet formulation by Liquid Chromatography, International Research Journal of Pharmacy, 1, (2), 135-141, 2011.

  • Mukesh M., Richa R., “Simultaneous estimation of Ambroxol Hydrochloride and Cetirizine Hydrochloride in tablet dosage form by RP-HPLC method,” International Journal of Comprehensive Pharmacy, 2, (03), 2010.

  • Rakeshkotkar P., Shirkhedkar A., and Sanjaysurana J., “Development and Validation of RP-HPLC method for simultaneous estimation of Cefpodoxime Proxetil and Ambroxol Hydrochloride in bulk and in tablets,” International Journal of Research in Pharmaceutical and Biomedical Sciences, 3 (1), 156- 164, 2012.

  • Baraddharam H., Badmanabhan R., “RP-HPLC method of Simultaneous Estimation of Gemifloxacin Mesylate and Ambroxol Hcl in combined dosage form,” International Journal of Research in Pharmacy and Chemistry, 1,(3), 379-385, 2011.

  • Krishnaveninagappan J., Meyyanathansn R., “A RP-HPLC method for Simultaneous Estimation of Ambroxol Hydrochloride and Loratidine in pharmaceutical formulation,” Research Journal Pharmceutical and Technology, 1, (4), 456-462, 2008.

  • Sunil P., Sudhakarbabu K., Navneet K., “Development and validation of Stability Indicating the RP-HPLC method for the estimation of related compounds of Guaifenesin in pharmaceutical dosage forms,” Pharmaceutical Methods, 2, (4), 374-380, 2011.

  • Abdilozdemir G., Halilaksoy A., “Determination of Guaifenesin and Dextromethorphan in a Cough Syrup by HPLC with Fluorometric detection,” International Journal of Pharmaceutical Sciences Review and Research, 51, (2), 117–122, 2006.

  • Senthilraja M., and Giriraj P., “Reverse Phase HPLC method for the simultaneous estimation of Terbutanile Sulphate, Bromhexine Hcl and Guaifenesin in Cough Syrup,” Asian Journal Pharmceutical Clinical Research, 4, (2), 1315 2011.

  • Giorgio G., Erika G., Robertarusso B., “Simultaneous, Stability Indicating HPLC-DAD determination of Guaifenesin and Methyl and Propyl Parabens in Cough Syrup,” Journal of Pharmaceutical and Biomedical Analysis, 41, 798–803 2007.

  • Narendranyola J., Govindasamyjeyabalan H., “Method development and validation of Levosalbutamol Sulphate in pure and tablet dosage form by RP-HPLC. “Journal of Applied Pharmaceutical Science,” 2, (6), 155-158, 2012.

  • Lakshmi P., Sathishkumarshetty H., “Simultaneous Estimation of Levosalbutamol Sulphate and Beclomethasone Dipropionate in combined Rota Cap dosage form BY RP-HPLC method “International Journal of Biological & Pharmaceutical Research,” 3, (3), 320-326, 2012.

  • Kim R., Sunil K., Prachi K., Kuldeep D., “Spectrophotometric simultaneous analysis of Ambroxol Hydrochloride, Guaifenesin and Terbutaline Sulphate in liquid dosage form (syrup)” International Journal of Pharmaceutical Sciences Review and Research, 8, (2), 15-23 2011.

  • Jigar G., Jasmin K., “Development and validation of Dual Wavelength UV Spectrophotometric method for simultaneous estimation of Ambroxol Hydrochloride and Cefpodoxime Proxetile in their combined tablet dosage form,” International Research Journal Of Pharmacy, 3 (4), 330-334, 2012.

  • Umadevi B., “Development and Validation of UV Spectrophotometric determination of Doxofylline and Ambroxol Hydrochloride In bulk and combined tablet formulation,” International Journal of Pharmaceutical Research, 3, (4), 89-92, 2005.

  • Amit P., Himasnshu D., Shedpure P., Sawant D., “Spectrophotometric simultaneous estimation of Salbutamol and Ambroxol In bulk and formulation “Asian Journal Pharmceutical Clinical Research, 4, (3), 4245-4251, 2011.

  • Prasanthi N., Mohanch K., Manikirans, N., “Estimation of Ambroxol Hydrochloride and Guiaphensin in tablet dosage form by simultaneous equation method,” International Journal of Research in Ayurveda & Pharmacy, 1, (1), 140-146, 2010.

  • Iangovanponnilavarasan H., “Simultaneous estimation of Ambroxol Hydrochloride and Loratadine in tablet dosage form by using UV Spectrophotometric method,” International Journal of Pharma and Bio Sciences, 2, (2), 338-345, 2006.

  • Sharma E., “Development and Validation of Dual Wavelength UV Spectrophotometric method for simultaneous estimation of Ambroxol Hydrochloride And Desloratadine Hydrochloride in their combined tablet dosage form,” International Journal of Pharmaceutical Research, 3,(8), 2584 –2590, 2012.

  • Nehal S., “Development and Validation of First Order Derivative Spectrophotometric method for simultaneous estimation of Ambroxol Hydrochloride and Desloratadine Hydrochloride in combined tablet dosage form “International Journal of Pharmaceutical Research and BioScience, 1, (2), 155-166, 2012.

  • Jigar G., Dr. Jagdish K., “Development and Validation of High Performance Thin Layer Chromatography method for simultaneous estimation of Ambroxol Hydrochloride and Cefpodoxime Proxetile in their combined tablet dosage form,” International Journal of Pharmaceutical Research and BioScience,1,(2), 143-154, 2012.

  • Jigar G., “Development and Validation of Q-Absorbance Ratio Method for Simultaneous Estimation of Ambroxol Hydrochloride and Cefpodoxime proxetile in combined tablet dosage form “International Journal of Pharma scholars, 1, (1), 56-62, 2012.

  • Perez T., Martinez C., Sanz A., Bravo E., “Determination of Bromhexine and Ambroxol in pharmaceutical dosage forms urine and blood serum.”, 692, 199-205, 1997.

  • Sahu R., Sharma Hemendra K., “Spectrophotometric determination of Guaifenesin and Pseudoephedrine Hydrochloride in tablet dosage form,” International Journal of Pharmaceutical Research, 1, (3), 41-49, 2011.

  • Sagarsuman P., Beravenkatavaraharavikumar B., “Difference UV Spectrophotometric method for estimation of Levosalbutamol Sulphate in tablet dosage form “Journal of Pharmaceutical Education Research, 3, (1), 17- 25, 2011.

  • Lakshmi P., Sathishkumarshetty H., “Simultaneous Estimation of Levosalbutamol Sulphate and Beclomethasone Dipropionate in combined Rota Cap dosage form BY RP-HPLC method “International Journal of Biological & Pharmaceutical Research,” 3, (3), 320-326, 2012.

  • Lakshmi P., Sathishkumarshetty H., “Development of new spectrophotometric methods for the simultaneous estimation of Levosalbutamol Sulphate and Beclomethasone Dipropionate in bulk drug and pharmaceutical formulations “International Journal of Pharmtech Research,”, 4, (2), 791-798, 2012.

  • Sharma E., “Development and validation of High Performance Thin Layer Chromatography method for simultaneous estimation of Ambroxol Hydrochloride and Desloratadine Hydrochloride in their combined tablet dosage form,” International Research Journal of Pharmacy, 3 (4), 305-309, 2012.

  • Prasant J., “Stability-Indicating HPTLC determination of Ambroxol Hydrochloride in bulk drug and pharmaceutical dosage form,” Journal of Chromatographic Science, 48, 45-49, 2010.


Abstract Views: 1667

PDF Views: 3




  • Spectrophotometric Estimation of Ambroxol Hydrochloride, Guaifenesin and Levosalbutamol Sulphatein Syrup

Abstract Views: 1667  |  PDF Views: 3

Authors

Nirav C. Patel
Department of Quality Assurance, Shree S. K. Patel College of Pharmaceutical Education and Research, Ganpat University, Ganpat Vidyanagar – 384012, Dist. – Mehsana (Gujarat), India
Dipti B. Patel
Department of Quality Assurance, Shree S. K. Patel College of Pharmaceutical Education and Research, Ganpat University, Ganpat Vidyanagar – 384012, Dist. – Mehsana (Gujarat), India
Pruthviraj K. Chaudhari
Department of Quality Assurance, Shree S. K. Patel College of Pharmaceutical Education and Research, Ganpat University, Ganpat Vidyanagar – 384012, Dist. – Mehsana (Gujarat), India

Abstract


This study proposes a method for estimation of Ambroxol HCl, Guaifenesin and Levosalbutamol sulphate in syrup form. The study was done by combining three spectrophotometric methods viz use of first order derivative and colorimetry. Absorption of Guaifenesin and Levosalbutamol sulphate were found to be zero at 323 nm, thus enabling the measurement of Ambroxol HCl, using specific absorbance in first order spectrum. Same way absorption of Ambroxol HCl and Levosalbutamol sulphate were found to be zero at 276 nm, thus enabling the measurement of Guaifenesin using specific absorbance in first order spectrum. For colorimetric measurement of Levosalbutamol Sulphate, a colored substance was obtained by coupling the oxidized product of Levosalbutamol sulphate with 4- aminoantipyrine and Potassium ferricyanide and its absorption was measured at 503 nm. The proposed method was statistically validated in accordance with ICH guidelines and results were found to be satisfactory for accuracy, precision and specificity.

Keywords


Ambroxol HCL, Guaifenesin And Levosalbutamol Sulphate, First Order Derivative, Colorimetric Method

References