Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Improved Production of L-Lysine by Immobilized Biotin Auxotropic Mutant Micrococcus glutamicus AB200


Affiliations
1 Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
     

   Subscribe/Renew Journal


Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-lysine using a biotin autotrophic mutant Micrococcus glutamicus AB<SUB>200</SUB> developed in laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB5. Under the same physico- chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-lysine. Production was increased significantly with cells entrapped in calcium alginate beads compared to free cells with the presence of 0.2(M) CaCl<SUB>2</SUB> in synthetic medium, 0.2 (M) CaCl<SUB>2</SUB> for bead formation, 4% sodium alginate, 24 h storage period of beads and 1.5 cell/alginate ratio but the accumulation of was decreased significantly with the whole cell entrapped in agar blocks, compared to free cells.

Keywords

Immobilization, L-lysine, Calcium Alginate Bead, Encapsulation, Mutant
Subscription Login to verify subscription
User
Notifications
Font Size


  • Sarva Rao, B., Muralidhararao and Swamy, AV.N., 2011 “Studies on Continuous production Kinetics of L-Lysine by a Immobilized Corynebacterium glutamicum” 13032, Middle-East Journal of Scientific Research, 7 (2): 235-240.

  • Ikeda M., Amino acid production process. Adv. Biochem. Eng/Biotechnol., 2003; 79; 1-35

  • Hackel U., Klein J., Megnet R., Wagner F. 1975 Immobilization of microbial cells in polymeric matrices. Eur. J. APPL. Microbiol., 1, 291-293.

  • Groboilot A., Boadi DK., Poncelet D. and Neufeld RJ, 1994, Immobilization of cells for application in the food industry. Crit. Rev. Biotechnol., 14: 75-107.

  • Prasad B. and Mishra IM., 1995, On the kinetics and effectiveness of immobilized whole cell batch culture. Biores. Technol., 53: 269-275.

  • Ganguly S., Banik A.K., Production of L-glutamic acid by Immobilized Micrococcus glutamicus AB100 Asian J. Research Chem. 4(6): June, 2011.

  • Gupta A, Das S.K and Banik A.K, Induced mutation and selection of a high yielding strain of Micrococcus glutamicus for L-lysine production. International Journal of Pharma and Bio Science. 3(2): 2012; B35-B42.

  • Beshay U., Production of alkaline protease by Terredinobacter turniae cells immobilized in calcium alginate beads. African Journal of biotechnology, 2, 2003, 60-65.

  • Nampoothiri K. M. and Panday A., Immobilization of Brevibacterium cells for the production of L-glutamic acid. Boi resource technology, 63,1998, 101-106.

  • Ganguly S. and Banik A.K. Production of L-glutamic acid by Immobilized Micrococcus Glutamicus AB100. Asian journal of research in chemistry.

  • Shah A.H., Hameed A, Ahmad S. and Khan GM. Optimization of culture condition for L-lysine fermentation by Corynebacterium glutamicum. Online Journal of Biological Science. 2(3); 2002: 151-156

  • Lu W.M. and Chem W.C., Production of L-glutamate using entrapped living cells of Bevibacterium ammoniagenes with calcium alginate gel, In proceedings of the national Science Council, Taipail, Taiwan, 6, 1988, 400-406.

  • Shimmyo A., Kimura H. and Okada H., Physiology of L-amylase production by immobilized Bacillus amyloliquefaciens. European Journal of Applied Microbiology Biotechnology.

  • Gosmann B. and Rehm HJ. Oxygen uptake of microorganisms entrapped in calcium alginate. Applied Microbiology and Biotechnokogy, 23, 1986, 163-167.

  • Ramkrishna S.V. and Prakasham R.S. Microbial fermentation with immobilized cells. Current Science, 87, 1999, 87-100.


Abstract Views: 364

PDF Views: 1




  • Improved Production of L-Lysine by Immobilized Biotin Auxotropic Mutant Micrococcus glutamicus AB200

Abstract Views: 364  |  PDF Views: 1

Authors

A. Gupta
Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
S. K. Das
Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
A. K. Banik
Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009

Abstract


Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-lysine using a biotin autotrophic mutant Micrococcus glutamicus AB<SUB>200</SUB> developed in laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB5. Under the same physico- chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-lysine. Production was increased significantly with cells entrapped in calcium alginate beads compared to free cells with the presence of 0.2(M) CaCl<SUB>2</SUB> in synthetic medium, 0.2 (M) CaCl<SUB>2</SUB> for bead formation, 4% sodium alginate, 24 h storage period of beads and 1.5 cell/alginate ratio but the accumulation of was decreased significantly with the whole cell entrapped in agar blocks, compared to free cells.

Keywords


Immobilization, L-lysine, Calcium Alginate Bead, Encapsulation, Mutant

References