Asian Journal of Research in Chemistry

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Gc- MS, FT-IR and NMR Spectroscopy Analysis for Metabolome Profiling of Thyme Oil


Affiliations
1 Department of Microbiology, Dharampeth M.P.Deo Memorial Science College, Nagpur, MS, India
2 Department of Microbiology, Sevadal Mahila Mahavidyalaya Nagpur, MS, India
     

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The tracking of phytoconstituents is an important step for isolation of biologically active compounds from vegetal source. In this study GC- MS, FT-IR and NMR spectroscopic tools were used to characterize various secondary metabolites of thyme oil. The information provided by various spectrophotometric data enabled the indepth characterisation of thyme oil. The phytochemical screening of thyme oil (TO) revealed the presence of Terpinene-4-ol (32.7%) followed by Thymol (18.1%), -Terpinene (7.4%), Carvacrol (5.6%) and -pinene (3.5%), but the levels of other compounds were low. FT -IR analysis mainly revealed the presence of Alkyl, Aromatic, Phenolic, Amines, Nitro, Aldehyde ,functional Groups and Alpha, and beta unsaturated aliphatic compounds. Particular emphasis has been given to the use of NMR as a fast and reliable tool to analyse thyme oil using 1H, 13C, NMR (on Bruker DRX- 300 spectrophotometer). The present study demonstrates that thyme oil metabolome profiling by GC-MS combined with FT-IR and NMR is useful for characterising the secondary metabolites of volatile essential oil.

Keywords

Thyme oil, metabolome profiling, GC-MS, FT-IR and NMR.
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  • Thompson JD, Manicacci D and Tarayre M. Thirty-five years of thyme: a tale of two polymorphisms. BioScience; 48(10); 1998: 805-15.

  • Stahl-Biskup E. and Saez F. Thyme. Taylor and Francis, London. 2002.

  • Koul O, Walia S and Dhaliwa G. Essential Oils as Green Pesticides: Potential and Constraints. Biopestic. Int. 4(1); 2008: 63–84.

  • Lambert RJW and Pearson. Susceptibility testing: accurate and reproducible minimum inhibitory concentration (NIC) values. Journal of Applied Microbiology, 91; 2000: 453-462.

  • Ultee A, Gorris LG and Smit EJ. Bactericidal activity of carvacrol towards the food-pathogen Bacillus Cereus. Journal Applied Microbiology, 85; 1998: 211-218.

  • Guillen MD and Manzanos MJ. Study of the composition of the different parts of Spanish Thymus vulgaris L. plants. Food Chem., 63; 1998: 373–383.

  • Jordán MJ, Martínez RM, Cases MA and Sotomayor JA. Watering level effect on Thymus hyemalis Lange essential oil yield and composition. J. Agric. Food Chem., 51; 2003: 5420– 5427.

  • Sotomayor JA, Martínez RM, García AJ and Jordán MJ. Thymus zygis subsp. Gracilis: watering level effect on phytomass production and essential oil quality. J. Agric. Food Chem., 52; 2004: 5418–5424.

  • Piccaglia RM, Marotti E, Giovanelli, Deans SG and Eaglesham E . Antibacterial and antioxidant properties of Mediterranean aromatic plants. Industrial Crops Prod., 2; 1993: 7–50.

  • Ozcan M and Chalchat JC. Aroma profile of thymus vulgaris L. Growing wild in turkey. Bulg. J. Plant Physiol., 30; 2004: 68-73.

  • Jordán MJ, Mart´inez RM, Goodner KL, Baldwin EA and Sotomayor JA. Seasonal variation of Thymus hyemalis Lange and Spanish Thymus vulgaris L. essential oils composition. Industrial Crops Prod., 24; 2006: 253–263.

  • Imelouane B, Amhamd H, Wathelet JP, Ankit M, Khedid K and Bachiri EL. Chemical Composition and Antimicrobial Activity of Essential Oil of Thyme (Thymus vulgaris) from Eastern Morocco.Int. J. Agric. Biol., 11( 2); 2009: 205–208.

  • Gouyon PH, Vernet PH, Guillerm JL and Valdeyron G. Polymorphisms and environment: the adaptative value of the oil polymorphisms in ¹Thymus vulgaris L. Heredity, 57; 1986: 59– 66.

  • Sturart BH. Infrared Spectroscopy: Fundamentals and Applications, John wiely and sons U. K. 2004.

  • Tomi F and Casanova J. 13CNMR as a tool for identification of individual components of essential oils from Labiatae-A review. Acta Horticult., 723; 2006: 185-192.

  • Salgueiro LR, Vila R, Tomi F, Figueiredo AC, Barroso JG, Cañigueral S, Casanova J, Cunha AP, Adzet T. Variability of essential oils of Thymus caespititius from Portugal. Phytochemistry. 45; 1997: 307–311.


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  • Gc- MS, FT-IR and NMR Spectroscopy Analysis for Metabolome Profiling of Thyme Oil

Abstract Views: 917  |  PDF Views: 2

Authors

Archana Kulkarni
Department of Microbiology, Dharampeth M.P.Deo Memorial Science College, Nagpur, MS, India
Nasreen Jan
Department of Microbiology, Dharampeth M.P.Deo Memorial Science College, Nagpur, MS, India
Seema Nimbarte
Department of Microbiology, Sevadal Mahila Mahavidyalaya Nagpur, MS, India

Abstract


The tracking of phytoconstituents is an important step for isolation of biologically active compounds from vegetal source. In this study GC- MS, FT-IR and NMR spectroscopic tools were used to characterize various secondary metabolites of thyme oil. The information provided by various spectrophotometric data enabled the indepth characterisation of thyme oil. The phytochemical screening of thyme oil (TO) revealed the presence of Terpinene-4-ol (32.7%) followed by Thymol (18.1%), -Terpinene (7.4%), Carvacrol (5.6%) and -pinene (3.5%), but the levels of other compounds were low. FT -IR analysis mainly revealed the presence of Alkyl, Aromatic, Phenolic, Amines, Nitro, Aldehyde ,functional Groups and Alpha, and beta unsaturated aliphatic compounds. Particular emphasis has been given to the use of NMR as a fast and reliable tool to analyse thyme oil using 1H, 13C, NMR (on Bruker DRX- 300 spectrophotometer). The present study demonstrates that thyme oil metabolome profiling by GC-MS combined with FT-IR and NMR is useful for characterising the secondary metabolites of volatile essential oil.

Keywords


Thyme oil, metabolome profiling, GC-MS, FT-IR and NMR.

References