A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Gupta, A.
- Improved Production of L-Lysine by Immobilized Biotin Auxotropic Mutant Micrococcus glutamicus AB200
Authors
1 Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
Source
Asian Journal of Research in Chemistry, Vol 6, No 7 (2013), Pagination: 613-617Abstract
Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-lysine using a biotin autotrophic mutant Micrococcus glutamicus AB<SUB>200</SUB> developed in laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB5. Under the same physico- chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-lysine. Production was increased significantly with cells entrapped in calcium alginate beads compared to free cells with the presence of 0.2(M) CaCl<SUB>2</SUB> in synthetic medium, 0.2 (M) CaCl<SUB>2</SUB> for bead formation, 4% sodium alginate, 24 h storage period of beads and 1.5 cell/alginate ratio but the accumulation of was decreased significantly with the whole cell entrapped in agar blocks, compared to free cells.Keywords
Immobilization, L-lysine, Calcium Alginate Bead, Encapsulation, MutantReferences
- Sarva Rao, B., Muralidhararao and Swamy, AV.N., 2011 “Studies on Continuous production Kinetics of L-Lysine by a Immobilized Corynebacterium glutamicum” 13032, Middle-East Journal of Scientific Research, 7 (2): 235-240.
- Ikeda M., Amino acid production process. Adv. Biochem. Eng/Biotechnol., 2003; 79; 1-35
- Hackel U., Klein J., Megnet R., Wagner F. 1975 Immobilization of microbial cells in polymeric matrices. Eur. J. APPL. Microbiol., 1, 291-293.
- Groboilot A., Boadi DK., Poncelet D. and Neufeld RJ, 1994, Immobilization of cells for application in the food industry. Crit. Rev. Biotechnol., 14: 75-107.
- Prasad B. and Mishra IM., 1995, On the kinetics and effectiveness of immobilized whole cell batch culture. Biores. Technol., 53: 269-275.
- Ganguly S., Banik A.K., Production of L-glutamic acid by Immobilized Micrococcus glutamicus AB100 Asian J. Research Chem. 4(6): June, 2011.
- Gupta A, Das S.K and Banik A.K, Induced mutation and selection of a high yielding strain of Micrococcus glutamicus for L-lysine production. International Journal of Pharma and Bio Science. 3(2): 2012; B35-B42.
- Beshay U., Production of alkaline protease by Terredinobacter turniae cells immobilized in calcium alginate beads. African Journal of biotechnology, 2, 2003, 60-65.
- Nampoothiri K. M. and Panday A., Immobilization of Brevibacterium cells for the production of L-glutamic acid. Boi resource technology, 63,1998, 101-106.
- Ganguly S. and Banik A.K. Production of L-glutamic acid by Immobilized Micrococcus Glutamicus AB100. Asian journal of research in chemistry.
- Shah A.H., Hameed A, Ahmad S. and Khan GM. Optimization of culture condition for L-lysine fermentation by Corynebacterium glutamicum. Online Journal of Biological Science. 2(3); 2002: 151-156
- Lu W.M. and Chem W.C., Production of L-glutamate using entrapped living cells of Bevibacterium ammoniagenes with calcium alginate gel, In proceedings of the national Science Council, Taipail, Taiwan, 6, 1988, 400-406.
- Shimmyo A., Kimura H. and Okada H., Physiology of L-amylase production by immobilized Bacillus amyloliquefaciens. European Journal of Applied Microbiology Biotechnology.
- Gosmann B. and Rehm HJ. Oxygen uptake of microorganisms entrapped in calcium alginate. Applied Microbiology and Biotechnokogy, 23, 1986, 163-167.
- Ramkrishna S.V. and Prakasham R.S. Microbial fermentation with immobilized cells. Current Science, 87, 1999, 87-100.
- Solid Phase Synthesis and Their Screening System-Review
Authors
1 National Institute of Ayurvedic Pharmaceutical Research, CCRAS, Dept. of Ayush, Patiala, IN
2 National Botanical Research Institute, Rana Pratap Marg, Lucknow, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 3 (2011), Pagination: 362-369Abstract
The use of solid phase synthesis techniques has been explored as an alternative to conventional approaches for the synthesis of compounds used in the drug discovery process. Combinatorial chemistry now plays an important role in the lead discovery and hit optimization processes in the pharmaceutical world as well as in other areas of discovery chemistry. Now days this is an important technique for the development of different synthesis concepts that were intended to cover and explore the chemical space without having to prepare every individual compound. Solid phase synthesis technologies were developed in response to the increased screening capacities and target oriented lead finding. This article explains different linker, protecting group, cleavage methods used in solid phase synthesis, combinatorial libraries and screening system used in solid phase synthesis.