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Syamasundar, B.
- Liquid Chromatographic Determination of Dobutamine HCL in Pharmaceutical Formulations
Authors
1 Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar – 522 510, Andhra Pradesh, IS
2 Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar – 522 510, Andhra Pradesh, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 2 (2011), Pagination: 304-307Abstract
The objective of the study is to develop a reversed phase high performance liquid chromatographic method using ultra violet detection for the determination of Dobutamine HCl in bulk and pharmaceutical dosage forms. Phenomenex Luna C18 column (250 mm x 4.6 mm, 5μ) was used as the stationary phase with a mixture of Acetonitrile:Methanol:Tetrahydrofuran (70:20:10 v/v/v) as the mobile phase. The response of the drug was linear in the concentration range of 10-30 μg/ml. Limit of detection and Limit of Quantification were found to be 0.34 and 1.1 μg/ml, respectively. The percentage recovery ranged between 99.54 and 100.06%. The factors affecting column separation of the analyte were studied. The results demonstrated that this method is reliable, reproducible and suitable for routine analysis.