Asian Journal of Research in Chemistry

Varun Dasari ¹, Bahlul Z. Awen ², Babu Rao Chandu ², Mukkanti Khagga ³, Nagaraju Pappula ¹

Affiliations
1 Hindu College of Pharmacy, Guntur-522002, Andhra Pradesh, IN
2 College of Pharmacy, Al-Jabal Al-Gharbi University, Al-Zawia, LY
3 Centre for Pharmacy, JNT University, Hyderabad, Andhra Pradesh, IN

Abstract

A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Ritonavir and Lopinavir in pharmaceutical dosage forms. The mobile phase consisted of Acetonitrile: buffer (0.05 M Potassium dihydrogen orthophosphate, P^H-3) in the ratio of 50:50 v/v delivered at a flow rate of 2.0 ml/min and wavelength of detection at 215 nm. The retention times of Ritonavir and Lopinavir were 10.253 min and 12.490 min respectively. The developed method was validated according to ICH guidelines. The proposed method can be used for determination of these drugs in combined dosage forms.

Keywords

Ritonavir, Lopinavir, RP-HPLC.

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