Asian Journal of Research in Chemistry

Rupali Kirtawade ¹, Pallavi Salve ¹, Chhotaram Seervi ¹, Kiran Patil ¹, Anita Kulkarni ¹, Pandurang Dhabale ¹

Affiliations
1 Govt. College of Pharmacy, Karad-415124, (Satara), Maharashtra, IN

Abstract

Two simple, accurate, precise, reproducible and economical procedures for simultaneous estimation of Atenolol (AT) and Losartan Potassium (LP) in tablet dosage form have been developed. First method based on solving of simultaneous equation using 226 nm (λmax of AT) and 208 nm (λmax of LP) as two analytical wavelengths for both drugs in Methanol solvent. Second method based on an equation of area calculation of curve at two wavelength region (231 to 221 nm and 213 to 203 nm). Linearity was observed in the concentration range of 6-36 μg/ml for AT and 2-16 μg/ml for LP. The results of analysis have been validated statistically and by recovery study.

Keywords

Atenolol, Losartan Potassium, λmax, Simultaneous Estimation Method, Area Under Curve (AUC).

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Chhotaram Seervi ¹, Kundan Pawar ¹, P. N. Dhabale ¹, I. D. Gonjari ¹, Chandrakant Raut ¹, Deepali Gharge ¹

Affiliations
1 Govt. College of Pharmacy, Karad-415124 (Satara), Maharashtra, IN

Abstract

Two simple, precise and economical UV methods have been developed for the estimation of Ambroxol Hydrochloride in bulk and pharmaceutical formulations. Ambroxol Hydrochloride has the absorbance maxima at 246.5 nm (Method A), and in the first order derivative spectra showed sharp peak at 240 nm (Method B). Linearity for detector response was observed in the concentration range of 10-50 μg/ml for all two methods. The proposed methods were successfully applied for the estimation of Ambroxol Hydrochloride in commercial pharmaceutical preparation by using simple solvent Distilled water. The results of the tablet analysis were validated with respect to accuracy (recovery), linearity, Limit of Detection and Limit of Quantification and specificity were found to be satisfactory.

Keywords

Ambroxol Hydrochloride, UV Spectrophotometry, Absorbance Maxima, Derivative Spectroscopy.

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