Asian Journal of Research in Chemistry

Tripti Jain ¹, Amber Vyas ², Darshan Dubey ³, Kamlesh Dashora ³, Vishal Jain ²

Affiliations
1 Chhattisgarh Food and Drug Administration, Mahasamund (CG)., IN
2 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.)., IN
3 Institute of Pharmacy, Vikram University, Ujjain (MP)., IN

Abstract

Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. Fingerprint method for Nilaavaarai Chooranam with TLC Densitometric Methods (HPTLC) using piperine as an internal standard was developed. The HPTLC estimation was carried out with three laboratory batches and one marketed formulation of Nilaavaarai Chooranam and crude drug containing piperine. The concentration of piperine present in raw material was found to be 2.9836±0.497w/w in Piper nigrum and in three identical laboratory batch of Nilaavaarai Chooranam NC-I, NC-II and N-III, was found to be 0.1761 ± 0.384, 0.1759 ± 0.534%, 0.1760 ± 0.019 w/w respectively. The piperine content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations. Hence the present method is simple, sensitive, precise and accurate and can be adopted for routine fingerprinting method for quality control of Nilaavaarai Chooranam.

Keywords

Nilaavaarai chooranam, Piperine, Sidhha formulation, HPTLC, Standardization.

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